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dc.contributor.authorCanario Viana, Marcus Viniciusen_US
dc.contributor.authorFigueiredo, Henriqueen_US
dc.contributor.authorRamos, Rommelen_US
dc.contributor.authorGuimaraes, Luis Carlosen_US
dc.contributor.authorPereira, Felipe Luizen_US
dc.contributor.authorDorella, Fernanda Alvesen_US
dc.contributor.authorSelim, Salah Abdel Karimen_US
dc.contributor.authorSalaheldean, Mohammaden_US
dc.contributor.authorSilva, Arturen_US
dc.contributor.authorWattam, Alice R.en_US
dc.contributor.authorAzevedo, Vascoen_US
dc.description.abstractCorynebacterium pseudotuberculosis is a Gram-positive, pleomorphic, facultative intracellular pathogen that causes Oedematous Skin Disease (OSD) in buffalo. To better understand the pathogenic mechanisms of OSD, we performed a comparative genomic analysis of 11 strains of C. pseudotuberculosis isolated from different buffalo found to be infected in Egypt during an outbreak that occurred in 2008. Sixteen previously described pathogenicity islands (PiCp) were present in all of the new buffalo strains, but one of them, PiCp12, had an insertion that contained both a corynephage and a diphtheria toxin gene, both of which may play a role in the adaptation of C. pseudotuberculosis to this new host. Synteny analysis showed variations in the site of insertion of the corynephage during the same outbreak. A gene functional comparison showed the presence of a nitrate reductase operon that included genes involved in molybdenum cofactor biosynthesis, which is necessary for a positive nitrate reductase phenotype and is a possible adaptation for intracellular survival. Genomes from the buffalo strains also had fusions in minor pilin genes in the spaA and spaD gene cluster (spaCX and spaYEF), which could suggest either an adaptation to this particular host, or mutation events in the immediate ancestor before this particular epidemic. A phylogenomic analysis confirmed a clear separation between the Ovis and Equi biovars, but also showed what appears to be a clustering by host species within the Equi strains.
dc.rightsAttribution 4.0en_US
dc.titleComparative genomic analysis between Corynebacterium pseudotuberculosis strains isolated from buffaloen_US
dc.typeArticle - Refereeden_US
dc.description.versionPeer Revieweden_US
dc.title.serialPLOS ONEen_US

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