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dc.contributor.authorLaimbeer, Francis Parker Effinghamen_US
dc.date.accessioned2018-08-03T08:01:00Z
dc.date.available2018-08-03T08:01:00Z
dc.date.issued2018-08-02
dc.identifier.othervt_gsexam:16698en_US
dc.identifier.urihttp://hdl.handle.net/10919/84480
dc.description.abstractInvestigations of potato (Solanum tuberosum) have been hampered by its complicated genetics and high genetic load. This dissertation applies genome reduction techniques to investigate a broad swath of genomic and physiological phenomena. It begins with the presentation and evaluation of a protocol to characterize endoreduplication within potato tubers, demonstrating substantial variation between tissue types and among wild species which may facilitate research into the genesis and growth of these starchy underground stems. Next, we transitioned to explore the distribution and consequences of a specific class of transposable element, Miniature Inverted Transposable Elements (MITEs), showing that they comprise approximately 5% of the potato genome, occur more frequently in genes with stress-related functions, and may be associated with changes, especially decreases, in gene expression. We then combined homology and sparsity based approaches to predict recent MITE activity, identifying five families as especially active. Finally, we expose the gene underlying the potato flower color locus, a homolog of AN2, while showing the effects it exerts on the flavonoid biosynthesis and fruit ripening pathways. This region was shown to be particularly dynamic, replete with MITEs and structural variants which we hypothesize to be the ultimate cause of differences in AN2 expression within the germplasm we examined. While the separate topics of this dissertation are quite disparate, each addresses an important topic in potato genetics, the in-depth study of which is only possible through the utilization of genomic reduction approaches to acquire homozygous genotypes for study and currently available genomic resources.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsThis item is protected by copyright and/or related rights. Some uses of this item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en_US
dc.subjectSolanum tuberosumen_US
dc.subjectpotatoen_US
dc.subjectendoreduplicationen_US
dc.subjectflow cytometryen_US
dc.subjectploidyen_US
dc.subjecttransposonsen_US
dc.subjectMITEsen_US
dc.subjectanthocyaninsen_US
dc.titlePotato genomics three ways: quantification of endoreduplication in tubers, a romp through the transposon terrain, and elucidation of flower color regulationen_US
dc.typeDissertationen_US
dc.contributor.departmentHorticultureen_US
dc.description.degreePh. D.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineHorticultureen_US
dc.contributor.committeechairVeilleux, Richard E.en_US
dc.contributor.committeememberSaghai-Maroof, Mohammad A.en_US
dc.contributor.committeememberBombarely Gomez, Aurelianoen_US
dc.contributor.committeememberTokuhisa, James G.en_US


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