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dc.contributor.authorPampalona, Juditen_US
dc.contributor.authorRoscioli, Emanueleen_US
dc.contributor.authorSilkworth, William T.en_US
dc.contributor.authorBowden, Brenten_US
dc.contributor.authorGenesca, Annaen_US
dc.contributor.authorTusell, Lauraen_US
dc.contributor.authorCimini, Danielaen_US
dc.date.accessioned2018-09-07T14:37:52Z
dc.date.available2018-09-07T14:37:52Z
dc.date.issued2016-01-19en_US
dc.identifier.othere0147420en_US
dc.identifier.urihttp://hdl.handle.net/10919/84972
dc.description.abstractAccurate chromosome segregation during cell division is essential to maintain genome stability, and chromosome segregation errors are causally linked to genetic disorders and cancer. An anaphase chromosome bridge is a particular chromosome segregation error observed in cells that enter mitosis with fused chromosomes/sister chromatids. The widely accepted Breakage/Fusion/Bridge cycle model proposes that anaphase chromosome bridges break during mitosis to generate chromosome ends that will fuse during the following cell cycle, thus forming new bridges that will break, and so on. However, various studies have also shown a link between chromosome bridges and aneuploidy and/or polyploidy. In this study, we investigated the behavior and properties of chromosome bridges during mitosis, with the idea to gain insight into the potential mechanism underlying chromosome bridge-induced aneuploidy. We find that only a small number of chromosome bridges break during anaphase, whereas the rest persist through mitosis into the subsequent cell cycle. We also find that the microtubule bundles (k-fibers) bound to bridge kinetochores are not prone to breakage/detachment, thus supporting the conclusion that k-fiber detachment is not the cause of chromosome bridge-induced aneuploidy. Instead, our data suggest that while the microtubules bound to the kinetochores of normally segregating chromosomes shorten substantially during anaphase, the k-fibers bound to bridge kinetochores shorten only slightly, and may even lengthen, during anaphase. This causes some of the bridge kinetochores/chromosomes to lag behind in a position that is proximal to the cell/spindle equator and may cause the bridged chromosomes to be segregated into the same daughter nucleus or to form a micronucleus.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherPLOSen_US
dc.rightsCreative Commons Attribution 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleChromosome Bridges Maintain Kinetochore-Microtubule Attachment throughout Mitosis and Rarely Break during Anaphaseen_US
dc.typeArticle - Refereeden_US
dc.description.versionPeer Revieweden_US
dc.title.serialPLOS ONEen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0147420en_US
dc.identifier.volume11en_US
dc.identifier.issue1en_US
dc.type.dcmitypeTexten_US
dc.identifier.pmid26784746en_US
dc.identifier.eissn1932-6203en_US


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Creative Commons Attribution 4.0
License: Creative Commons Attribution 4.0