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dc.contributor.authorRoy, Adhirajen_US
dc.contributor.authorDement, Angela D.en_US
dc.contributor.authorCho, Kyu Hongen_US
dc.contributor.authorKim, Jeong-Hoen_US
dc.date.accessioned2018-09-28T14:02:02Z
dc.date.available2018-09-28T14:02:02Z
dc.date.issued2015-03-27en_US
dc.identifier.othere0121985en_US
dc.identifier.urihttp://hdl.handle.net/10919/85184
dc.description.abstractThe transport of glucose across the plasma membrane is mediated by members of the glucose transporter family. In this study, we investigated glucose uptake through the yeast hexose transporter 1 (Hxt1) by measuring incorporation of 2-NBDG, a non-metabolizable, fluorescent glucose analog, into the yeast Saccharomyces cerevisiae. We find that 2-NBDG is not incorporated into the hxt null strain lacking all glucose transporter genes and that this defect is rescued by expression of wild type Hxt1, but not of Hxt1 with mutations at the putative glucose-binding residues, inferred from the alignment of yeast and human glucose transporter sequences. Similarly, the growth defect of the hxt null strain on glucose is fully complemented by expression of wild type Hxt1, but not of the mutant Hxt1 proteins. Thus, 2-NBDG, like glucose, is likely to be transported into the yeast cells through the glucose transport system. Hxt1 is internalized and targeted to the vacuole for degradation in response to glucose starvation. Among the mutant Hxt1 proteins, Hxt1N370A and HXT1W473A are resistant to such degradation. Hxt1N370A, in particular, is able to neither uptake 2-NBDG nor restore the growth defect of the hxt null strain on glucose. These results demonstrate 2-NBDG as a fluorescent probe for glucose uptake in the yeast cells and identify N370 as a critical residue for the stability and function of Hxt1.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherPLOSen_US
dc.rightsCreative Commons Attribution 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleAssessing Glucose Uptake through the Yeast Hexose Transporter 1 (Hxt1)en_US
dc.typeArticle - Refereeden_US
dc.description.versionPeer Revieweden_US
dc.title.serialPLOS ONEen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0121985en_US
dc.identifier.volume10en_US
dc.identifier.issue3en_US
dc.type.dcmitypeTexten_US
dc.identifier.pmid25816250en_US
dc.identifier.eissn1932-6203en_US


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License: Creative Commons Attribution 4.0