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dc.contributor.authorZhang, Jileien
dc.contributor.authorWei, Lanjingen
dc.contributor.authorKelly, Patricken
dc.contributor.authorFreeman, Marken
dc.contributor.authorJaegerson, Kirstenen
dc.contributor.authorGong, Jiansenen
dc.contributor.authorXu, Buen
dc.contributor.authorPan, Zhimingen
dc.contributor.authorXu, Chuanlingen
dc.contributor.authorWang, Chengmingen
dc.description.abstractTo facilitate the detection of Salmonella and to be able to rapidly and conveniently determine the species/subspecies present, we developed and tested a generic and differential FRET-PCR targeting their tetrathionate reductase response regulator gene. The differential pan-Salmonella FRET-PCR we developed successfully detected seven plasmids that contained partial sequences of S. bongori and the six S. enterica subspecies. The detection limit varied from ∼5 copies of target gene/per PCR reaction for S. enterica enterica to ∼200 for S. bongori. Melting curve analysis demonstrated a Tm of ∼68°C for S. enterica enterica, ∼62.5°C for S. enterica houtenae and S. enterica diarizonae, ∼57°C for S. enterica indica, and ∼54°C for S. bongori, S. enterica salamae and S. enterica arizonae. The differential pan-Salmonella FRET-PCR also detected and determined the subspecies of 4 reference strains and 47 Salmonella isolated from clinically ill birds or pigs. Finally, we found it could directly detect and differentiate Salmonella in feline (5/50 positive; 10%; one S. enterica salamae and 4 S. enterica enterica) and canine feces (15/114 positive; 13.2%; all S. enterica enterica). The differential pan-Salmonella FRET-PCR failed to react with 96 non-Salmonella bacterial strains. Our experiments show the differential pan-Salmonella FRET-PCR we developed is a rapid, sensitive and specific method to detect and differentiate Salmonella.en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.titleDetection of Salmonella spp. Using a Generic and Differential FRET-PCRen
dc.typeArticle - Refereeden
dc.description.versionPeer Revieweden
dc.title.serialPLOS ONEen

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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International