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dc.contributor.authorSha, Weien
dc.contributor.authorMartins, Ana M.en
dc.contributor.authorLaubenbacher, Reinhard C.en
dc.contributor.authorMendes, Pedroen
dc.contributor.authorShulaev, Vladimiren
dc.date.accessioned2018-10-17T14:56:40Zen
dc.date.available2018-10-17T14:56:40Zen
dc.date.issued2013-09-20en
dc.identifier.otherUNSP e74939en
dc.identifier.urihttp://hdl.handle.net/10919/85394en
dc.description.abstractOxidative stress is a well-known biological process that occurs in all respiring cells and is involved in pathophysiological processes such as aging and apoptosis. Oxidative stress agents include peroxides such as hydrogen peroxide, cumene hydroperoxide, and linoleic acid hydroperoxide, the thiol oxidant diamide, and menadione, a generator of superoxide, amongst others. The present study analyzed the early temporal genome-wide transcriptional response of Saccharomyces cerevisiae to oxidative stress induced by the aromatic peroxide cumene hydroperoxide. The accurate dataset obtained, supported by the use of temporal controls, biological replicates and well controlled growth conditions, provided a detailed picture of the early dynamics of the process. We identified a set of genes previously not implicated in the oxidative stress response, including several transcriptional regulators showing a fast transient response, suggesting a coordinated process in the transcriptional reprogramming. We discuss the role of the glutathione, thioredoxin and reactive oxygen species-removing systems, the proteasome and the pentose phosphate pathway. A data-driven clustering of the expression patterns identified one specific cluster that mostly consisted of genes known to be regulated by the Yap1p and Skn7p transcription factors, emphasizing their mediator role in the transcriptional response to oxidants. Comparison of our results with data reported for hydrogen peroxide identified 664 genes that specifically respond to cumene hydroperoxide, suggesting distinct transcriptional responses to these two peroxides. Genes up-regulated only by cumene hydroperoxide are mainly related to the cell membrane and cell wall, and proteolysis process, while those down-regulated only by this aromatic peroxide are involved in mitochondrial function.en
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleThe Genome-Wide Early Temporal Response of Saccharomyces cerevisiae to Oxidative Stress Induced by Cumene Hydroperoxideen
dc.typeArticle - Refereeden
dc.description.versionPeer Revieweden
dc.title.serialPLOS ONEen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0074939en
dc.identifier.volume8en
dc.identifier.issue9en
dc.type.dcmitypeTexten
dc.identifier.pmid24073228en
dc.identifier.eissn1932-6203en


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International