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dc.contributor.authorOttesen, Andrea R.en_US
dc.contributor.authorGonzalez, Antonioen_US
dc.contributor.authorBell, Rebeccaen_US
dc.contributor.authorArce, Carolineen_US
dc.contributor.authorRideout, Stevenen_US
dc.contributor.authorAllard, Marcen_US
dc.contributor.authorEvans, Peteren_US
dc.contributor.authorStrain, Errolen_US
dc.contributor.authorMusser, Stevenen_US
dc.contributor.authorKnight, Roben_US
dc.contributor.authorBrown, Ericen_US
dc.contributor.authorPettengill, James B.en_US
dc.date.accessioned2018-10-17T14:56:40Z
dc.date.available2018-10-17T14:56:40Z
dc.date.issued2013-09-09en_US
dc.identifier.othere73079en_US
dc.identifier.urihttp://hdl.handle.net/10919/85396
dc.description.abstractThe ability to detect a specific organism from a complex environment is vitally important to many fields of public health, including food safety. For example, tomatoes have been implicated numerous times as vehicles of foodborne outbreaks due to strains of Salmonella but few studies have ever recovered Salmonella from a tomato phyllosphere environment. Precision of culturing techniques that target agents associated with outbreaks depend on numerous factors. One important factor to better understand is which species co-enrich during enrichment procedures and how microbial dynamics may impede or enhance detection of target pathogens. We used a shotgun sequence approach to describe taxa associated with samples pre-enrichment and throughout the enrichment steps of the Bacteriological Analytical Manual's (BAM) protocol for detection of Salmonella from environmental tomato samples. Recent work has shown that during efforts to enrich Salmonella (Proteobacteria) from tomato field samples, Firmicute genera are also co-enriched and at least one co-enriching Firmicute genus (Paenibacillus sp.) can inhibit and even kills strains of Salmonella. Here we provide a baseline description of microflora that co-culture during detection efforts and the utility of a bioinformatic approach to detect specific taxa from metagenomic sequence data. We observed that uncultured samples clustered together with distinct taxonomic profiles relative to the three cultured treatments (Universal Pre-enrichment broth (UPB), Tetrathionate (TT), and Rappaport-Vassiliadis (RV)). There was little consistency among samples exposed to the same culturing medias, suggesting significant microbial differences in starting matrices or stochasticity associated with enrichment processes. Interestingly, Paenibacillus sp. (Salmonella inhibitor) was significantly enriched from uncultured to cultured (UPB) samples. Also of interest was the sequence based identification of a number of sequences as Salmonella despite indication by all media, that samples were culture negative for Salmonella. Our results substantiate the nascent utility of metagenomic methods to improve both biological and bioinformatic pathogen detection methods.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherPLOSen_US
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleCo-Enriching Microflora Associated with Culture Based Methods to Detect Salmonella from Tomato Phyllosphereen_US
dc.typeArticle - Refereeden_US
dc.description.versionPeer Revieweden_US
dc.contributor.departmentVirginia Agricultural Experiment Stationen_US
dc.title.serialPLOS ONEen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0073079en_US
dc.identifier.volume8en_US
dc.identifier.issue9en_US
dc.type.dcmitypeTexten_US
dc.identifier.pmid24039862en_US
dc.identifier.eissn1932-6203en_US


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International