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dc.contributor.authorBittleman, Katelyn Rose
dc.contributor.authorDong, Shuping
dc.contributor.authorRoman, Maren
dc.contributor.authorLee, Yong Woo
dc.date.accessioned2018-11-09T15:02:41Z
dc.date.available2018-11-09T15:02:41Z
dc.date.issued2018-10-24
dc.identifier.issn2470-1343
dc.identifier.urihttp://hdl.handle.net/10919/85811
dc.description.abstractThe study evaluates cellulose nanocrystals (CNCs) as nanocarriers for targeted, intracellular delivery of molecular agents. CNCs were labeled with fluorescein-5′-isothiocyanate as an imaging agent and conjugated to folic acid (FA) as a targeting ligand. The CNC conjugates were characterized by UV–vis spectroscopy, ζ-potential analysis, dynamic light scattering, and atomic force microscopy. Cellular binding/uptake of the FA-conjugated CNCs by KB and MDA-MB-468 cells was quantified with cellular uptake assays. Internalization of the particles was confirmed by confocal microscopy. Uptake mechanisms were determined by inhibition studies with chlorpromazine and genistein. Binding affinity was qualitatively assessed with a free folate inhibition assay. Both KB and MDA-MB-468 cells exhibited significant and folate-receptor specific binding/uptake of FA-conjugated CNCs. Clathrin-mediated endocytosis was a significant uptake mechanism in both cell types, whereas caveolae-mediated endocytosis only played a significant role in MDA-MB-468 cells. Uptake inhibition of FA-conjugated CNCs by KB cells required high concentrations (>1 mM) of free FA. The observed FR-specific internalization of FA-conjugated CNCs by FR-positive cancer cells and tumors and their remarkable high affinity for the FR demonstrate the great potential of CNCs as novel nanocarriers for imaging agents and chemotherapeutics in the early detection and treatment of cancer.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsCreative Commons Attribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectDrug delivery systemsen_US
dc.subjectFluorescence imagingen_US
dc.subjectImaging agentsen_US
dc.subjectNanocrystalsen_US
dc.subjectPharmacologyen_US
dc.subjectProteinsen_US
dc.titleFolic Acid-Conjugated Cellulose Nanocrystals Show High Folate-Receptor Binding Affinity and Uptake by KB and Breast Cancer Cellsen_US
dc.typeArticle - Refereeden_US
dc.title.serialACS Omegaen_US
dc.identifier.doihttps://doi.org/10.1021/acsomega.8b01619
dc.identifier.volume3en_US
dc.identifier.issue10en_US
dc.type.dcmitypeText


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Creative Commons Attribution-NonCommercial 4.0 International
License: Creative Commons Attribution-NonCommercial 4.0 International