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dc.contributor.authorDai, Rujuanen_US
dc.contributor.authorZhang, Yanen_US
dc.contributor.authorKhan, Deenaen_US
dc.contributor.authorHeid, Bettinaen_US
dc.contributor.authorCaudell, Daviden_US
dc.contributor.authorCrasta, Oswalden_US
dc.contributor.authorAhmed, S. Ansaren_US
dc.date.accessioned2018-11-09T20:22:02Z
dc.date.available2018-11-09T20:22:02Z
dc.date.issued2010-12-10en_US
dc.identifier.othere14302en_US
dc.identifier.urihttp://hdl.handle.net/10919/85815
dc.description.abstractBackground Recent reports have shown that microRNAs (miRNAs) regulate vital immunological processes and have emerged as key regulators of immune system development and function. Therefore, it is important to determine miRNA dysregulation and its pathogenic contribution in autoimmune diseases, an aspect not adequately addressed thus far. Methodology/Principal Findings In this study, we profiled miRNA expressions in splenic lymphocytes from three murine lupus models (MRL-lpr, B6-lpr and NZB/WF1) with different genetic background by miRNA microarray assays and Real-time RT-PCR. Despite the genetic differences among these three lupus stains, a common set of dysregulated miRNAs (miR-182-96-183 cluster, miR-31, and miR-155) was identified in splenocytes when compared with age-matched control mice. The association of these miRNAs with the disease was highlighted by our observation that this miRNA expression pattern was evident in NZB/W mice only at an age when lupus disease is manifested. Further, we have shown that the miRNA dysregulation in MRL-lpr mice was not simply due to the activation of splenocytes. By Real-time RT-PCR, we confirmed that these miRNAs were upregulated in both purified splenic B and T cells from MRL-lpr mice. miR-127 and miR-379, which were greatly upregulated in splenocytes from lpr mice, were moderately increased in diseased NZB/W mice. In addition, Real-time RT-PCR revealed that miR-146a, miR-101a, and miR-17-92 were also markedly upregulated in splenic T, but not B cells from MRL-lpr mice. Conclusions/Significance The identification of common lupus disease-associated miRNAs now forms the basis for the further investigation of the pathogenic contribution of these miRNAs in autoimmune lupus, which will advance our knowledge of the role of miRNAs in autoimmunity. Given that miRNAs are conserved, with regard to both evolution and function, our observation of a common lupus disease-associated miRNA expression pattern in murine lupus models is likely to have significant pathogenic, diagnostic, and/or therapeutic implications in human lupus.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherPLOSen_US
dc.rightsCreative Commons Attribution 4.0 Internationalen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleIdentification of a Common Lupus Disease-Associated microRNA Expression Pattern in Three Different Murine Models of Lupusen_US
dc.typeArticle - Refereeden_US
dc.description.versionPeer Revieweden_US
dc.title.serialPLOS ONEen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0014302en_US
dc.identifier.volume5en_US
dc.identifier.issue12en_US
dc.type.dcmitypeTexten_US
dc.identifier.pmid21170274en_US
dc.identifier.eissn1932-6203en_US


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License: Creative Commons Attribution 4.0 International