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dc.contributor.authorMoustafa, Dina A.en
dc.contributor.authorJain, Neetaen
dc.contributor.authorSriranganathan, Nammalwaren
dc.contributor.authorVemulapalli, Rameshen
dc.date.accessioned2018-11-09T20:22:02Zen
dc.date.available2018-11-09T20:22:02Zen
dc.date.issued2010-11-24en
dc.identifier.othere14112en
dc.identifier.urihttp://hdl.handle.net/10919/85816en
dc.description.abstractBrucella neotomae is not known to be associated with clinical disease in any host species. Previous research suggested that B. neotomae might not express detectable levels of Cu/Zn superoxide dismutase (SOD), a periplasmic enzyme known to be involved in protecting Brucella from oxidative bactericidal effects of host phagocytes. This study was undertaken to investigate the genetic basis for the disparity in SOD expression in B. neotomae. Our Western blot and SOD enzyme assay analyses indicated that B. neotomae does express SOD, but at a substantially reduced level. Nucleotide sequence analysis of region upstream to the sodC gene identified a single-nucleotide insertion in the potential promoter region. The same single-nucleotide insertion was also detected in the sodC promoter of B. suis strain Thomsen, belonging to biovar 2 in which SOD expression was undetectable previously. Examination of the sodC promoter activities using translational fusion constructs with E. coli β-galactosidase demonstrated that the B. neotomae and B. suis biovar 2 promoters were very weak in driving gene expression. Site-directed mutation studies indicated that the insertion of A in the B. neotomae sodC promoter reduced the promoter activity. Increasing the level of SOD expression in B. neotomae through complementation with B. abortus sodC gene did not alter the bacterial survival in J774A.1 macrophage-like cells and in tissues of BALB/c and C57BL/6 mice. These results for the first time demonstrate the occurrence of a single-nucleotide polymorphism affecting promoter function and gene expression in Brucella.en
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIdentification of a Single-Nucleotide Insertion in the Promoter Region Affecting the sodC Promoter Activity in Brucella neotomaeen
dc.typeArticle - Refereeden
dc.description.versionPeer Revieweden
dc.title.serialPLOS ONEen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0014112en
dc.identifier.volume5en
dc.identifier.issue11en
dc.type.dcmitypeTexten
dc.identifier.pmid21124845en
dc.identifier.eissn1932-6203en


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Creative Commons Attribution 4.0 International
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