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dc.contributor.authorZhao, Bingyu
dc.contributor.authorDahlbeck, Douglas
dc.contributor.authorKrasileva, Ksenia V.
dc.contributor.authorFong, Richard W.
dc.contributor.authorStaskawicz, Brian J.
dc.date.accessioned2018-12-04T18:42:15Z
dc.date.available2018-12-04T18:42:15Z
dc.date.issued2011-12-01
dc.identifier.issn1553-7366
dc.identifier.othere1002408
dc.identifier.urihttp://hdl.handle.net/10919/86223
dc.description.abstractEffectors of the bacterial type III secretion system provide invaluable molecular probes to elucidate the molecular mechanisms of plant immunity and pathogen virulence. In this report, we focus on the AvrBs2 effector protein from the bacterial pathogen Xanthomonas euvesicatoria (Xe), the causal agent of bacterial spot disease of tomato and pepper. Employing homology-based structural analysis, we generate a three-dimensional structural model for the AvrBs2 protein and identify catalytic sites in its putative glycerolphosphodiesterase domain (GDE). We demonstrate that the identified catalytic region of AvrBs2 was able to functionally replace the GDE catalytic site of the bacterial glycerophosphodiesterase BhGlpQ cloned from Borrelia hermsii and is required for AvrBs2 virulence. Mutations in the GDE catalytic domain did not disrupt the recognition of AvrBs2 by the cognate plant resistance gene Bs2. In addition, AvrBs2 activation of Bs2 suppressed subsequent delivery of other Xanthomonas type III effectors into the host plant cells. Investigation of the mechanism underlying this modulation of the type III secretion system may offer new strategies to generate broad-spectrum resistance to bacterial pathogens.en_US
dc.description.sponsorshipThis work was funded by the Two Blades Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.format.extent13 pages
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherPLOS
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectcampestris pv. vesicatoriaen_US
dc.subjectnontypable haemophilus-influenzaeen_US
dc.subjectiii secretion systemsen_US
dc.subjectbs3 resistance geneen_US
dc.subjectglycerophosphodiester phosphodiesteraseen_US
dc.subjectpseudomonas-syringaeen_US
dc.subjectbacterial spoten_US
dc.subjectprotein-den_US
dc.subjectnicotiana-benthamianaen_US
dc.subjectdisease resistanceen_US
dc.titleComputational and Biochemical Analysis of the Xanthomonas Effector AvrBs2 and Its Role in the Modulation of Xanthomonas Type Three Effector Deliveryen_US
dc.typeArticle - Refereed
dc.title.serialPLOS Pathogens
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1002408
dc.identifier.volume7
dc.identifier.issue12
dc.type.dcmitypeText
dc.identifier.pmid22144898
dc.identifier.eissn1553-7374


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International