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dc.contributor.authorKim, Ji Hyun
dc.contributor.authorSeol, Young-Joon
dc.contributor.authorKo, In Kap
dc.contributor.authorKang, Hyun-Wook
dc.contributor.authorLee, Young Koo
dc.contributor.authorYoo, James J.
dc.contributor.authorAtala, Anthony
dc.contributor.authorLee, Sang Jin
dc.date.accessioned2018-12-11T17:55:43Z
dc.date.available2018-12-11T17:55:43Z
dc.date.issued2018-08-17
dc.identifier.issn2045-2322
dc.identifier.other12307
dc.identifier.urihttp://hdl.handle.net/10919/86342
dc.description.abstractA bioengineered skeletal muscle tissue as an alternative for autologous tissue flaps, which mimics the structural and functional characteristics of the native tissue, is needed for reconstructive surgery. Rapid progress in the cell-based tissue engineering principle has enabled in vitro creation of cellularized muscle-like constructs; however, the current fabrication methods are still limited to build a three-dimensional (3D) muscle construct with a highly viable, organized cellular structure with the potential for a future human trial. Here, we applied 3D bioprinting strategy to fabricate an implantable, bioengineered skeletal muscle tissue composed of human primary muscle progenitor cells (hMPCs). The bioprinted skeletal muscle tissue showed a highly organized multi-layered muscle bundle made by viable, densely packed, and aligned myofiber-like structures. Our in vivo study presented that the bioprinted muscle constructs reached 82% of functional recovery in a rodent model of tibialis anterior (TA) muscle defect at 8 weeks of post-implantation. In addition, histological and immunohistological examinations indicated that the bioprinted muscle constructs were well integrated with host vascular and neural networks. We demonstrated the potential of the use of the 3D bioprinted skeletal muscle with a spatially organized structure that can reconstruct the extensive muscle defects.en_US
dc.description.sponsorshipWake Forest Clinical and Translational Science Institute [UL1 TR001420]; Army; Navy; NIH; Air Force; VA; Health Affairs [W81XWH-14-2-0004]; U.S. Army Medical Research Acquisition Activity, Fort Detrick MD [21702-5014]; Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science, and Technology [2012R1A6A3A03040684]
dc.format.extent15 pages
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherSpringer Nature
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectengineered muscle
dc.subjectin-vitro
dc.subjectloss injury
dc.subjectrat model
dc.subjecttissue
dc.subjectcell
dc.subjectregeneration
dc.subjecthydrogel
dc.subjectmyotubes
dc.subjectvivo
dc.title3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restorationen_US
dc.typeArticle - Refereed
dc.description.notesWe thank H. S. Kim, J. S. Lee, and T. Bledsoe for a surgical procedure, Regenerative Medicine Clinical Center (RMCC) for hMPCs isolation, M. Devarasetty for imaging, and Y. M. Ju for technical assistance. The authors thank K. Klein at the Wake Forest Clinical and Translational Science Institute (UL1 TR001420) for editorial assistance. This work was supported by the Army, Navy, NIH, Air Force, VA and Health Affairs to support the AFIRM II effort under Award No. W81XWH-14-2-0004. The U.S. Army Medical Research Acquisition Activity, 820 Chandler Street, Fort Detrick MD 21702-5014 is the awarding and administering acquisition office. Opinions, interpretations, conclusions, and recommendations are those of the author and are not necessarily endorsed by the Department of Defense. J.H.K. was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2012R1A6A3A03040684).
dc.title.serialScientific Reports
dc.identifier.doihttps://doi.org/10.1038/s41598-018-29968-5
dc.identifier.volume8
dc.type.dcmitypeText
dc.identifier.pmid30120282


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Creative Commons Attribution 4.0 International
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