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dc.contributor.authorKim, Ji Hyunen
dc.contributor.authorSeol, Young-Joonen
dc.contributor.authorKo, In Kapen
dc.contributor.authorKang, Hyun-Wooken
dc.contributor.authorLee, Young Kooen
dc.contributor.authorYoo, James J.en
dc.contributor.authorAtala, Anthonyen
dc.contributor.authorLee, Sang Jinen
dc.date.accessioned2018-12-11T17:55:43Zen
dc.date.available2018-12-11T17:55:43Zen
dc.date.issued2018-08-17en
dc.identifier.issn2045-2322en
dc.identifier.other12307en
dc.identifier.urihttp://hdl.handle.net/10919/86342en
dc.description.abstractA bioengineered skeletal muscle tissue as an alternative for autologous tissue flaps, which mimics the structural and functional characteristics of the native tissue, is needed for reconstructive surgery. Rapid progress in the cell-based tissue engineering principle has enabled in vitro creation of cellularized muscle-like constructs; however, the current fabrication methods are still limited to build a three-dimensional (3D) muscle construct with a highly viable, organized cellular structure with the potential for a future human trial. Here, we applied 3D bioprinting strategy to fabricate an implantable, bioengineered skeletal muscle tissue composed of human primary muscle progenitor cells (hMPCs). The bioprinted skeletal muscle tissue showed a highly organized multi-layered muscle bundle made by viable, densely packed, and aligned myofiber-like structures. Our in vivo study presented that the bioprinted muscle constructs reached 82% of functional recovery in a rodent model of tibialis anterior (TA) muscle defect at 8 weeks of post-implantation. In addition, histological and immunohistological examinations indicated that the bioprinted muscle constructs were well integrated with host vascular and neural networks. We demonstrated the potential of the use of the 3D bioprinted skeletal muscle with a spatially organized structure that can reconstruct the extensive muscle defects.en
dc.description.sponsorshipWake Forest Clinical and Translational Science Institute [UL1 TR001420]; Army; Navy; NIH; Air Force; VA; Health Affairs [W81XWH-14-2-0004]; U.S. Army Medical Research Acquisition Activity, Fort Detrick MD [21702-5014]; Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science, and Technology [2012R1A6A3A03040684]en
dc.format.extent15 pagesen
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectengineered muscleen
dc.subjectin-vitroen
dc.subjectloss injuryen
dc.subjectrat modelen
dc.subjecttissueen
dc.subjectcellen
dc.subjectregenerationen
dc.subjecthydrogelen
dc.subjectmyotubesen
dc.subjectvivoen
dc.title3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restorationen
dc.typeArticle - Refereeden
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.description.notesWe thank H. S. Kim, J. S. Lee, and T. Bledsoe for a surgical procedure, Regenerative Medicine Clinical Center (RMCC) for hMPCs isolation, M. Devarasetty for imaging, and Y. M. Ju for technical assistance. The authors thank K. Klein at the Wake Forest Clinical and Translational Science Institute (UL1 TR001420) for editorial assistance. This work was supported by the Army, Navy, NIH, Air Force, VA and Health Affairs to support the AFIRM II effort under Award No. W81XWH-14-2-0004. The U.S. Army Medical Research Acquisition Activity, 820 Chandler Street, Fort Detrick MD 21702-5014 is the awarding and administering acquisition office. Opinions, interpretations, conclusions, and recommendations are those of the author and are not necessarily endorsed by the Department of Defense. J.H.K. was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2012R1A6A3A03040684).en
dc.title.serialScientific Reportsen
dc.identifier.doihttps://doi.org/10.1038/s41598-018-29968-5en
dc.identifier.volume8en
dc.type.dcmitypeTexten
dc.identifier.pmid30120282en


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Creative Commons Attribution 4.0 International
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