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dc.contributor.authorLuo, Chaochao
dc.contributor.authorZhao, Shengguo
dc.contributor.authorZhang, Muchen
dc.contributor.authorGao, Yanan
dc.contributor.authorWang, Jiaqi
dc.contributor.authorHanigan, Mark D.
dc.contributor.authorZheng, Nan
dc.date.accessioned2018-12-13T19:40:51Z
dc.date.available2018-12-13T19:40:51Z
dc.date.issued2018-03-02
dc.identifier.issn2045-2322
dc.identifier.other3912
dc.identifier.urihttp://hdl.handle.net/10919/86380
dc.description.abstractAmino acids (AA) are one of the key nutrients that regulate cell proliferation and casein synthesis in cow mammary epithelial cells (CMEC), but the mechanism of this regulation is not yet clear. In this study, the effect of SESN2 on AA-mediated cell proliferation and casein synthesis in CMEC was assessed. After 12 h of AA starvation, CMECs were cultured in the absence of all AA (AA-), in the presences of only essential AA (EAA+), or of all AA (AA+). Cell proliferation, casein expression, and activation of the mammalian target of rapamycin complex 1 (mTORC1) pathway were increased; but SESN2 expression was decreased in response to increased EAA or AA supply. Overexpressing or inhibiting SESN2 demonstrated that cell proliferation, casein expression, and activation of the mTORC1 pathway were all controlled by SESN2 expression. Furthermore, the increase in cell proliferation, casein expression, and activation of the mTORC1 pathway in response to AA supply was inhibited by overexpressing SESN2, and those effects were reversed by inhibiting SESN2. These results indicate that SESN2 is an important inhibitor of mTORC1 in CMEC blocking AA-mediated cell proliferation and casein synthesis.en_US
dc.description.sponsorshipSpecial Fund for Agro-scientific Research in the Public Interest [201403071]; Agricultural Science and Technology Innovation Program [ASTIP-ISA12]; Project of Risk Assessment on Raw Milk [GJFP2016008]
dc.format.extent10 pages
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherSpringer Nature
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjecttransfer-rna synthetase
dc.subjectmilk protein-synthesis
dc.subjectdairy-cow
dc.subjectmolecular-mechanisms
dc.subjectcycle progression
dc.subjectgene-expression
dc.subjectactivation
dc.subjectsestrins
dc.subjectleucine
dc.subjectstress
dc.titleSESN2 negatively regulates cell proliferation and casein synthesis by inhibition the amino acid-mediated mTORC1 pathway in cow mammary epithelial cellsen_US
dc.typeArticle - Refereed
dc.description.notesThis study was financially supported by the Special Fund for Agro-scientific Research in the Public Interest (201403071), the Agricultural Science and Technology Innovation Program (ASTIP-ISA12), and the Project of Risk Assessment on Raw Milk (GJFP2016008).
dc.title.serialScientific Reports
dc.identifier.doihttps://doi.org/10.1038/s41598-018-22208-w
dc.identifier.volume8
dc.type.dcmitypeText
dc.identifier.pmid29500367


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International