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dc.contributor.authorChandler, Morgan
dc.contributor.authorLyalina, Tatiana
dc.contributor.authorHalman, Justin
dc.contributor.authorRackley, Lauren
dc.contributor.authorLee, Lauren
dc.contributor.authorDang, Dylan
dc.contributor.authorKe, Weina
dc.contributor.authorSajja, Sameer
dc.contributor.authorWoods, Steven
dc.contributor.authorAcharya, Shrija
dc.contributor.authorBaumgarten, Elijah
dc.contributor.authorChristopher, Jonathan
dc.contributor.authorElshalia, Emman
dc.contributor.authorHrebien, Gabriel
dc.contributor.authorKublank, Kinzey
dc.contributor.authorSaleh, Saja
dc.contributor.authorStallings, Bailey
dc.contributor.authorTafere, Michael
dc.contributor.authorStriplin, Caryn
dc.contributor.authorAfonin, Kirill A.
dc.identifier.citationChandler, M.; Lyalina, T.; Halman, J.; Rackley, L.; Lee, L.; Dang, D.; Ke, W.; Sajja, S.; Woods, S.; Acharya, S.; Baumgarten, E.; Christopher, J.; Elshalia, E.; Hrebien, G.; Kublank, K.; Saleh, S.; Stallings, B.; Tafere, M.; Striplin, C.; Afonin, K.A. Broccoli Fluorets: Split Aptamers as a User-Friendly Fluorescent Toolkit for Dynamic RNA Nanotechnology. Molecules 2018, 23, 3178.
dc.description.abstractRNA aptamers selected to bind fluorophores and activate their fluorescence offer a simple and modular way to visualize native RNAs in cells. Split aptamers which are inactive until the halves are brought within close proximity can become useful for visualizing the dynamic actions of RNA assemblies and their interactions in real time with low background noise and eliminated necessity for covalently attached dyes. Here, we design and test several sets of F30 Broccoli aptamer splits, that we call fluorets, to compare their relative fluorescence and physicochemical stabilities. We show that the splits can be simply assembled either through one-pot thermal annealing or co-transcriptionally, thus allowing for direct tracking of transcription reactions via the fluorescent response. We suggest a set of rules that enable for the construction of responsive biomaterials that readily change their fluorescent behavior when various stimuli such as the presence of divalent ions, exposure to various nucleases, or changes in temperature are applied. We also show that the strand displacement approach can be used to program the controllable fluorescent responses in isothermal conditions. Overall, this work lays a foundation for the future development of dynamic systems for molecular computing which can be used to monitor real-time processes in cells and construct biocompatible logic gates.
dc.rightsCreative Commons Attribution 4.0 International
dc.titleBroccoli Fluorets: Split Aptamers as a User-Friendly Fluorescent Toolkit for Dynamic RNA Nanotechnologyen_US
dc.typeArticle - Refereed

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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International