Show simple item record

dc.contributor.authorHu, Wanqi
dc.contributor.authorTu, Zhijian J
dc.date.accessioned2019-01-02T12:55:34Z
dc.date.available2019-01-02T12:55:34Z
dc.date.issued2018-12-24
dc.identifier.citationParasites & Vectors. 2018 Dec 24;11(Suppl 2):655
dc.identifier.urihttp://hdl.handle.net/10919/86538
dc.description.abstractAbstract Background Aedes aegypti is an important mosquito vector that transmits arboviruses that cause devastating diseases including Zika, dengue fever, yellow fever and chikungunya. Improved understanding of gene regulation in the early development of Ae. aegypti will facilitate genetic studies and help the development of novel control strategies of this important disease vector. Results In this study, we demonstrated through transgenic assays that the promoter of an endogenous early zygotic gene KLC2 could drive gene expression in the syncytial blastoderm and early cellular blastoderm, which is a stage that the developing germline and the rest of embryo are accessible to genetic manipulation. An unexpected expression of the reporter gene in transgenic male testes was also observed. Further analysis confirmed the expression of the endogenous KLC2 in the testes, which was not detected in the previous RNA sequencing data. Conclusions Our finding provided a new promoter element that can be used in future genetic studies and applications in Ae. aegypti. Moreover, our transgenic reporter assays showed that cautions are needed when interpreting RNA sequencing data as transient or tissue-specific transcription may go undetected by RNAseq.
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.rightsCreative Commons Attribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleFunctional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegyptien_US
dc.typeArticle - Refereed
dc.date.updated2018-12-30T04:44:11Z
dc.description.versionPeer Reviewed
dc.rights.holderThe Author(s).
dc.title.serialParasites & Vectors
dc.identifier.doihttps://doi.org/10.1186/s13071-018-3210-0
dc.type.dcmitypeText


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International