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dc.contributor.authorThomason, Courtney A.
dc.contributor.authorMullen, Nathan
dc.contributor.authorBelden, Lisa K.
dc.contributor.authorMay, Meghan
dc.contributor.authorHawley, Dana M.
dc.date.accessioned2019-01-02T15:41:30Z
dc.date.available2019-01-02T15:41:30Z
dc.date.issued2017-11-23
dc.identifier.issn2045-2322
dc.identifier.other16177
dc.identifier.urihttp://hdl.handle.net/10919/86544
dc.description.abstractThere is growing evidence that symbiotic microbes play key roles in host defense, but less is known about how symbiotic microbes mediate pathogen-induced damage to hosts. Here, we use a natural wildlife disease system, house finches and the conjunctival bacterial pathogen Mycoplasma gallisepticum (MG), to experimentally examine the impact of the ocular microbiome on host damage and pathogen virulence factors during infection. We disrupted the ocular bacterial community of healthy finches using an antibiotic that MG is intrinsically resistant to, then inoculated antibiotic and sham-treated birds with MG. House finches with antibiotic-disrupted ocular microbiomes had more severe MG-induced conjunctival inflammation than birds with unaltered microbiomes, even after accounting for differences in conjunctival MG load. Furthermore, MG cultures from finches with disrupted microbiomes had increased sialidase enzyme and cytadherence activity, traits associated with enhanced virulence in Mycoplasmas, relative to isolates from sham-treated birds. Variation in sialidase activity and cytadherence among isolates was tightly linked with degree of tissue inflammation in hosts, supporting the consideration of these traits as virulence factors in this system. Overall, our results suggest that microbial dysbiosis can result in enhanced virulence of colonizing pathogens, with critical implications for the health of wildlife, domestic animals, and humans.en_US
dc.description.sponsorshipNIH as part of the joint NIH-NSF-USDA Ecology and Evolution of Infectious Diseases program [5R01GM105245]; NSF grant [DEB-1136640]
dc.format.extent8 pages
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherSpringer Nature
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectmycoplasma-gallisepticum infection
dc.subjectin-house finches
dc.subjectimmune-system
dc.subjectdisease
dc.subjectbacteria
dc.subjectsusceptibility
dc.subjectconjunctivitis
dc.subjectcytadherence
dc.subjecttransmission
dc.subjectsurvival
dc.titleResident Microbiome Disruption with Antibiotics Enhances Virulence of a Colonizing Pathogenen_US
dc.typeArticle - Refereed
dc.description.notesThis work was funded by NIH grant 5R01GM105245 (to DMH) as part of the joint NIH-NSF-USDA Ecology and Evolution of Infectious Diseases program and NSF grant DEB-1136640 to LKB. We thank Laila Kirkpatrick, Jeni Walke, and Courtney Pearson for technical assistance, and Ariel Leon, Sahnzi Moyers, Matt Aberle, Eddie Schuler, Natalie Bales, Courtney Youngbar, Ashlyn Garnish, Sarah Taylor, Morgan Villa, and Michal Vinkler for assistance with sample collection. Finally, we thank David Ley for providing isolates and the rest of the House Finch project team for useful feedback.
dc.title.serialScientific Reports
dc.identifier.doihttps://doi.org/10.1038/s41598-017-16393-3
dc.identifier.volume7
dc.type.dcmitypeText
dc.identifier.pmid29170421


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