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dc.contributor.authorZahid, Osama K.
dc.contributor.authorZhao, Boxuan Simen
dc.contributor.authorHe, Chuan
dc.contributor.authorHall, Adam R.
dc.date.accessioned2019-01-23T14:27:28Z
dc.date.available2019-01-23T14:27:28Z
dc.date.issued2016-07-07
dc.identifier.issn2045-2322
dc.identifier.other29565
dc.identifier.urihttp://hdl.handle.net/10919/86846
dc.description.abstract5-hydroxymethylcytosine (5 hmC), the oxidized form of 5-methylcytosine (5 mC), is a base modification with emerging importance in biology and disease. However, like most epigenetic elements, it is transparent to many conventional genetic techniques and is thus challenging to probe. Here, we report a rapid solid-state nanopore assay that is capable of resolving 5 hmC with high specificity and sensitivity and demonstrate its utility in assessing global modification abundance in genomic DNA.en_US
dc.description.sponsorshipWake Forest Baptist Comprehensive Cancer Center's NCI Cancer Center [P30CA012197]; NIH [1R21CA193067]; Sigma Xi [489441]; 3 M Non-tenured Faculty Award program
dc.format.extent6
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherSpringer Nature
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject5-hydroxymethylcytosine
dc.subject5-formylcytosine
dc.subject5-methylcytosine
dc.subjectdifferentiation
dc.subjectquantification
dc.subjectmicrornas
dc.subjectproteins
dc.subjectbrain
dc.titleQuantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanoporesen_US
dc.typeArticle - Refereed
dc.description.notesThe authors wish to thank the Howarth Lab (Oxford University) for supplying monovalent streptavidinand acknowledge services from the Wake Forest Cancer Genomics Shared Resource, supported by the Wake Forest Baptist Comprehensive Cancer Center's NCI Cancer Center Support Grant P30CA012197. This work was supported by NIH grant 1R21CA193067. O.K.Z. received support from the Sigma Xi Grants-in-Aid of research program (Grant ID No. 489441). A.R.H. acknowledges support from the 3 M Non-tenured Faculty Award program.
dc.title.serialScientific Reports
dc.identifier.doihttps://doi.org/10.1038/srep29565
dc.identifier.volume6
dc.type.dcmitypeText
dc.identifier.pmid27383905


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International