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dc.contributor.authorSolivio, Morwena J.
dc.contributor.authorLess, Rebekah
dc.contributor.authorRynes, Mathew L.
dc.contributor.authorKramer, Marcus
dc.contributor.authorAksan, Alptekin
dc.date.accessioned2019-01-23T14:27:29Z
dc.date.available2019-01-23T14:27:29Z
dc.date.issued2016-04-12
dc.identifier.issn2045-2322
dc.identifier.other24186
dc.identifier.urihttp://hdl.handle.net/10919/86850
dc.description.abstractDespite abundant research conducted on cancer biomarker discovery and validation, to date, less than two-dozen biomarkers have been approved by the FDA for clinical use. One main reason is attributed to inadvertent use of low quality biospecimens in biomarker research. Most proteinaceous biomarkers are extremely susceptible to pre-analytical factors such as collection, processing, and storage. For example, cryogenic storage imposes very harsh chemical, physical, and mechanical stresses on biospecimens, significantly compromising sample quality. In this communication, we report the development of an electrospun lyoprotectant matrix and isothermal vitrification methodology for non-cryogenic stabilization and storage of liquid biospecimens. The lyoprotectant matrix was mainly composed of trehalose and dextran (and various low concentration excipients targeting different mechanisms of damage), and it was engineered to minimize heterogeneity during vitrification. The technology was validated using five biomarkers; LDH, CRP, PSA, MMP-7, and C3a. Complete recovery of LDH, CRP, and PSA levels was achieved post-rehydration while more than 90% recovery was accomplished for MMP-7 and C3a, showing promise for isothermal vitrification as a safe, efficient, and low-cost alternative to cryogenic storage.en_US
dc.description.sponsorshipNIH-NCI grant [5R21CA157298]
dc.format.extent14
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherSpringer Nature
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectc-reactive protein
dc.subjectlactate-dehydrogenase
dc.subjectinduced denaturation
dc.subjectbiomarker discovery
dc.subjectcold denaturation
dc.subjectfrozen state
dc.subjectstabilization
dc.subjectcancer
dc.subjectblood
dc.subjectvitrification
dc.titleAdsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Seraen_US
dc.typeArticle - Refereed
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen_US
dc.description.notesThe authors thank Ms. Goeun Heo for her valuable assistance with electrospinning. This research was supported by an NIH-NCI grant (5R21CA157298) to A.A.
dc.title.serialScientific Reports
dc.identifier.doihttps://doi.org/10.1038/srep24186
dc.identifier.volume6
dc.type.dcmitypeText
dc.identifier.pmid27068126


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Creative Commons Attribution 4.0 International
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