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dc.contributor.authorRossmeisl, John H., Jr.
dc.contributor.authorGarcia, Paulo A.
dc.contributor.authorRobertson, John L.
dc.contributor.authorEllis, Thomas L.
dc.contributor.authorDavalos, Rafael V.
dc.date.accessioned2019-01-31T16:06:37Z
dc.date.available2019-01-31T16:06:37Z
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10919/87097
dc.description.abstractThis study describes the neuropathologic features of normal canine brain ablated with non-thermal irreversible electroporation (N-TIRE). The parietal cerebral cortices of four dogs were treated with N-TIRE using a dose-escalation protocol with an additional dog receiving sham treatment. Animals were allowed to recover following N-TIRE ablation and the effects of treatment were monitored with clinical and magnetic resonance imaging examinations. Brains were subjected to histopathologic and ultrastructural assessment along with Bcl-2, caspase-3, and caspase-9 immunohistochemical staining following sacrifice 72 h post-treatment. Adverse clinical effects of N-TIRE were only observed in the dog treated at the upper energy tier. MRI and neuropathologic examinations indicated that N-TIRE ablation resulted in focal regions of severe cytoarchitectural and blood-brain-barrier disruption. Lesion size correlated to the intensity of the applied electrical field. N-TIRE-induced lesions were characterized by parenchymal necrosis and hemorrhage; however, large blood vessels were preserved. A transition zone containing parenchymal edema, perivascular inflammatory cuffs, and reactive gliosis was interspersed between the necrotic focus and normal neuropil. Apoptotic labeling indices were not different between the N-TIRE-treated and control brains. This study identified N-TIRE pulse parameters that can be used to safely create circumscribed foci of brain necrosis while selectively preserving major vascular structures.en_US
dc.description.sponsorshipThis work was supported in part by the Wallace Coulter and National Science Foundations (NSF-CBET 0933335). The authors thank Dr. Bernard Jortner, Dr. Elankumaran Subbiah, Ms. Jennifer Rudd, and Mrs. Barbara Wheeler for technical assistance with the ultrastructural and immunohistochemical studies. Angiodynamics, Inc. (USA) provided the NanoKnife pulse generator and electrodes used in this study.
dc.format.extent8 pages
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherKorean Society of Veterinary Science
dc.rightsCreative Commons Attribution-NonCommercial 4.0
dc.rights.urihttp://www.creativecommons.org/licenses/by-nc/4.0/
dc.subjectcentral nervous system
dc.subjectdog
dc.subjectirreversible electroporation
dc.subjectneuropathology
dc.titlePathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brainen_US
dc.typeArticle - Refereed
dc.title.serialJournal of Veterinary Science
dc.identifier.doihttp://dx.doi.org/10.4142/jvs.2013.14.4.433
dc.identifier.volume14
dc.identifier.issue4
dc.type.dcmitypeText


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