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dc.contributor.authorYablonovitch, Arielle L.
dc.contributor.authorFu, Jeremy
dc.contributor.authorLi, Kexin
dc.contributor.authorMahato, Simpla
dc.contributor.authorKang, Lin
dc.contributor.authorRashkovetsky, Eugenia
dc.contributor.authorKorol, Abraham B.
dc.contributor.authorTang, Hua
dc.contributor.authorMichalak, Pawel
dc.contributor.authorZelhof, Andrew C.
dc.contributor.authorNevo, Eviatar
dc.contributor.authorLi, Jin Billy
dc.date.accessioned2019-02-07T14:44:22Z
dc.date.available2019-02-07T14:44:22Z
dc.date.issued2017-11-17
dc.identifier.issn2041-1723
dc.identifier.other1570
dc.identifier.urihttp://hdl.handle.net/10919/87524
dc.description.abstractDetermining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster from 'Evolution Canyon' in Israel, which consists of two opposing slopes with divergent microclimates. We identify several hundred differentially expressed genes and dozens of differentially edited sites between flies from each slope, correlate these changes with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin regulates its editing levels. We also demonstrate that while temperature affects editing levels at more sites than genetic differences, genetically regulated sites tend to be less affected by temperature. This work shows the extent to which gene expression and RNA editing differ between flies from different microclimates, and provides insights into the regulation responsible for these differences.en_US
dc.description.sponsorshipNIH [T32 GM008294, R21EY024125, R01 GM102484, R01 GM124215, R01 MH115080]
dc.description.sponsorshipBinational USA-Israel Science Foundation Grant BSF [2011438]
dc.description.sponsorshipResearch Foundation of Ancell-Teicher for Genetics and Molecular Evolution
dc.description.sponsorshipEllison Medical Foundation
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherSpringer Nature
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleRegulation of gene expression and RNA editing in Drosophila adapting to divergent microclimatesen_US
dc.typeArticle - Refereed
dc.description.notesWe thank members of the Li lab for comments, as well as Hunter Fraser, Tom Clandinin, Joe Lipsick, and Stephen Montgomery for helpful discussions. We also thank Drew Stenesen and Helmut Kramer for assistance with phenotyping flies. This work was supported by an NIH Molecular Biophysics Predoctoral Research Training Grant T32 GM008294 (A.L.Y.), Binational USA-Israel Science Foundation Grant BSF # 2011438 ( K.L., E.R., A.B.K., E.N.), NIH grant R21EY024125 (S.M. and A.C.Z.), Research Foundation of Ancell-Teicher for Genetics and Molecular Evolution (E.N.), and NIH grants R01 GM102484, R01 GM124215, and R01 MH115080 and the Ellison Medical Foundation (J.B.L.).
dc.title.serialNature Communications
dc.identifier.doihttps://doi.org/10.1038/s41467-017-01658-2
dc.identifier.volume8
dc.type.dcmitypeText
dc.identifier.pmid29146998


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Creative Commons Attribution 4.0 International
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