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dc.date.accessioned2019-02-26T22:23:57Z
dc.date.available2019-02-26T22:23:57Z
dc.date.issued2017-08-15
dc.identifier.urihttp://hdl.handle.net/10919/87816
dc.description.abstractAn integrated microfluidic chromatin immunoprecipitation assay dramatically improves the collection efficiency of ChIP DNA from cells. Immunoprecipitation of chromatin fragments is conducted in a microfluidic chamber with a large fraction of its volume (e.g., ˜15-40%) occupied by magnetic immunoprecipitation (IP) beads. Oscillating washing of the beads, enabled by, e.g., solenoid valves (controlled by a computer) and high pressure attached to both ends of the microfluidic chamber, effectively removes unbound chromatin and produces high-quality ChIP DNA. ChIP DNA produced by an example device generates excellent results in the subsequent DNA library preparation. The ChIP-seq (i.e., ChIP followed by next-generation sequencing) results match very well with public data generated using much larger cell sample sizes and a conventional approach.
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherUnited States Patent and Trademark Office
dc.titleMicrofluidic systems and methods for chromatin immunoprecipitation (ChIP)
dc.typePatent
dc.identifier.urlhttp://pimg-fpiw.uspto.gov/fdd/77/323/097/0.pdf
dc.date.accessed2019-02-15
dc.type.dcmitypeText
dc.contributor.assigneeVirginia Tech Intellectual Properties, Inc.
dc.contributor.inventorLu, Chang
dc.contributor.inventorCao, Zhenning
dc.date.filed2014-10-10
dc.identifier.applicationnumber14511422
dc.subject.cpcB01L3/50276
dc.subject.cpcB01L2200/0668
dc.subject.cpcB01L2300/0816
dc.subject.cpcB01L2300/0877
dc.subject.cpcB01L2400/043
dc.subject.cpcB01L2400/0487
dc.subject.cpcC12Q1/6806
dc.subject.cpcC12Q2522/10
dc.subject.cpcC12Q2565/629
dc.subject.cpcG01N33/54326
dc.subject.cpcG01N33/6875
dc.type.patenttypeutility
dc.identifier.patentnumber9732377


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