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dc.contributor.authorBardi, Gina T.
dc.contributor.authorAl-Rayan, Numan
dc.contributor.authorRichie, Jamaal L.
dc.contributor.authorYaddanapudi, Kavitha
dc.contributor.authorHood, Joshua L.
dc.date.accessioned2019-03-18T12:29:22Z
dc.date.available2019-03-18T12:29:22Z
dc.date.issued2019-03-12
dc.identifier.citationBardi, G.T.; Al-Rayan, N.; Richie, J.L.; Yaddanapudi, K.; Hood, J.L. Detection of Inflammation-Related Melanoma Small Extracellular Vesicle (sEV) mRNA Content Using Primary Melanocyte sEVs as a Reference. Int. J. Mol. Sci. 2019, 20, 1235.
dc.identifier.urihttp://hdl.handle.net/10919/88473
dc.description.abstractMelanoma-derived small extracellular vesicles (sEVs) participate in tumor pathogenesis. Tumor pathogenesis is highly dependent on inflammatory processes. Given the potential for melanoma sEVs to carry tumor biomarkers, we explored the hypothesis that they may contain inflammation-related mRNA content. Biophysical characterization showed that human primary melanocyte-derived sEVs trended toward being smaller and having less negative (more neutral) zeta potential than human melanoma sEVs (A-375, SKMEL-28, and C-32). Using primary melanocyte sEVs as the control population, RT-qPCR array results demonstrated similarities and differences in gene expression between melanoma sEV types. Upregulation of pro-angiogenic chemokine ligand CXCL1, CXCL2, and CXCL8 mRNAs in A-375 and SKMEL-28 melanoma sEVs was the most consistent finding. This paralleled increased production of CXCL1, CXCL2, and CXCL8 proteins by A-375 and SKMEL-28 sEV source cells. Overall, the use of primary melanocyte sEVs as a control sEV reference population facilitated the detection of inflammation-related melanoma sEV mRNA content.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherMDPI
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleDetection of Inflammation-Related Melanoma Small Extracellular Vesicle (sEV) mRNA Content Using Primary Melanocyte sEVs as a Referenceen_US
dc.typeArticle - Refereed
dc.date.updated2019-03-15T13:55:45Z
dc.title.serialInternational Journal of Molecular Science
dc.identifier.doihttps://doi.org/10.3390/ijms20051235
dc.type.dcmitypeText


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International