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dc.contributor.authorMcCutcheon, Kelly
dc.contributor.authorBandara, Aloka B.
dc.contributor.authorZuo, Ziwei
dc.contributor.authorHeflin, James R.
dc.contributor.authorInzana, Thomas J.
dc.date.accessioned2019-05-31T11:52:05Z
dc.date.available2019-05-31T11:52:05Z
dc.date.issued2019-05-21
dc.identifier.citationMcCutcheon, K.; Bandara, A.B.; Zuo, Z.; Heflin, J.R.; Inzana, T.J. The Application of a Nanomaterial Optical Fiber Biosensor Assay for Identification of Brucella Nomenspecies. Biosensors 2019, 9, 64.en
dc.identifier.urihttp://hdl.handle.net/10919/89653
dc.description.abstractBacteria in the genus Brucella are the cause of brucellosis in humans and many domestic and wild animals. A rapid and culture-free detection assay to detect Brucella in clinical samples would be highly valuable. Nanomaterial optical fiber biosensors (NOFS) are capable of recognizing DNA hybridization events or other analyte interactions with high specificity and sensitivity. Therefore, a NOFS assay was developed to detect Brucella DNA from cultures and in tissue samples from infected mice. An ionic self-assembled multilayer (ISAM) film was coupled to a long-period grating optical fiber, and a nucleotide probe complementary to the Brucella IS711 region and modified with biotin was bound to the ISAM by covalent conjugation. When the ISAM/probe duplex was exposed to lysate containing ≥100 killed cells of Brucella, or liver or spleen tissue extracts from Brucella-infected mice, substantial attenuation of light transmission occurred, whereas exposure of the complexed fiber to non-Brucella gram-negative bacteria or control tissue samples resulted in negligible attenuation of light transmission. Oligonucleotide probes specific for B. abortus, B. melitensis, and B. suis could also be used to detect and differentiate these three nomenspecies. In summary, the NOFS biosensor assay detected three nomenspecies of Brucella without the use of polymerase chain reaction within 30 min and could specifically detect low numbers of this bacterium in clinical samples.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherMDPI
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe Application of a Nanomaterial Optical Fiber Biosensor Assay for Identification of Brucella Nomenspeciesen_US
dc.typeArticle - Refereed
dc.date.updated2019-05-31T06:01:59Z
dc.title.serialBiosensors
dc.identifier.doihttps://doi.org/10.3390/bios9020064
dc.type.dcmitypeText


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International