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dc.contributor.authorPowers, Craigen
dc.contributor.authorHanlon, Reginaen
dc.contributor.authorSchmale, David G. IIIen
dc.identifier.citationPowers C, Hanlon R, Schmale III DG. 2018. Remote collection of microorganisms at two depths in a freshwater lake using an unmanned surface vehicle (USV) PeerJ 6:e4290 https://doi.org/10.7717/peerj.4290en
dc.description.abstractMicroorganisms are ubiquitous in freshwater aquatic environments, but little is known about their abundance, diversity, and transport. We designed and deployed a remote-operated water-sampling system onboard an unmanned surface vehicle (USV, a remote-controlled boat) to collect and characterize microbes in a freshwater lake in Virginia, USA. The USV collected water samples simultaneously at 5 and 50 cm below the surface of the water at three separate locations over three days in October, 2016. These samples were plated on a non-selective medium (TSA) and on a medium selective for the genus Pseudomonas (KBC) to estimate concentrations of culturable bacteria in the lake. Mean concentrations ranged from 134 to 407 CFU/mL for microbes cultured on TSA, and from 2 to 8 CFU/mL for microbes cultured on KBC. There was a significant difference in the concentration of microbes cultured on KBC across three sampling locations in the lake (P = 0.027), suggesting an uneven distribution of Pseudomonas across the locations sampled. There was also a significant difference in concentrations of microbes cultured on TSA across the three sampling days (P = 0.038), demonstrating daily fluctuations in concentrations of culturable bacteria. There was no significant difference in concentrations of microbes cultured on TSA (P = 0.707) and KBC (P = 0.641) across the two depths sampled, suggesting microorganisms were well-mixed between 5 and 50 cm below the surface of the water. About 1 percent (7/720) of the colonies recovered across all four sampling missions were ice nucleation active (ice+) at temperatures warmer than — 10 °C. Our work extends traditional manned observations of aquatic environments to unmanned systems, and highlights the potential for USVs to understand the distribution and diversity of microbes within and above freshwater aquatic environments.en
dc.description.sponsorshipNational Science Foundationen
dc.description.sponsorship(NSF): DEB-1241068, DGE-0966125, AGS-1520825, IIS-1637915.en
dc.format.extent16 pagesen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.subjectEcosystem Scienceen
dc.subjectFreshwater Biologyen
dc.subjectAquatic microbiologyen
dc.subjectPseudomonas syringaeen
dc.subjectUnmanned surface vehicleen
dc.subjectIce nucleationen
dc.subjectAir water interfaceen
dc.titleRemote collection of microorganisms at two depths in a freshwater lake using an unmanned surface vehicle (USV)en
dc.typeArticle - Refereeden
dc.contributor.departmentCivil and Environmental Engineeringen_US
dc.contributor.departmentSchool of Plant and Environmental Sciencesen_US

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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International