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dc.contributor.authorJeantet, Meriella Anita
dc.date.accessioned2019-07-03T20:33:54Z
dc.date.available2019-07-03T20:33:54Z
dc.date.issued1985
dc.identifier.urihttp://hdl.handle.net/10919/91126
dc.description.abstractThe present studies were conducted to gain a better understanding of the effects of pregnenolone (P₅), human chorionic gonadotropin (hCG) and 3' 5', cyclic adenosine monophosphate ( cAMP) on porcine placental and endometrial production of progesterone (P₄), testosterone (T) and estrone (E₁) at 30, 60 and 90 days of gestation. Duplicate 300 mg samples of placenta, endometrium or both (co-incubation) were incubated in medium199 containing either no P₅, P₅, P₅ + hCG or P₅ + cAMP for either zero (control), .5, 1 or 2 h. The first study compared P₄ and E₁ production with or without addition of P₅. At d 30, 60 and 90, respectively, P₄ production (ng/g) increased significantly in the presence (vs absence) of P₅ in the incubation medium of placental (13.2 vs 7.5, 73.9 vs 42.7, 137.4 vs 113.5, respectively) coincubation (14.5 vs 10.0, 33.6 vs 22.3, 77.9 vs 49.4, respectively) and endometrial (16.0 vs 13.3, 23.0 vs 16.0, 17.1 vs 6.7, respectively) tissue. Presence of P₅ increased E₁ production in d 60 (1.3 vs .7 ng/g) and d 90 (51.7 vs 34.6 ng/g) placental tissue and d 90 endometrial tissue (9.8 vs 8.0 ng/g). In a second study, P₅ + cAMP increased (vs P₅ alone) P₄ in placental tissue at d 30 (11.6 vs 8.7 ng/g) and coincubation tissue at d 90 (103.7 vs 75.3 ng/g). Cyclic AMP stimulated increased P₄ synthesis ( vs P₅ alone), throughout the incubation period in d 60 and d 90 tissue. E₁ production by endometrial tissue at d 30 (4.1 vs 2.9 ng/g), and placental tissue at d 60 ( 1. 2 vs . 9 ng/g). Presence of hCG in the incubation medium had no overall effect on either P₄ or E₁ accumulation. Only trace amounts of T were detected in either study, suggesting rapid aromatization of C₁₉ steroids to estrogens.en
dc.format.extentxiii, 146 leaves
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherVirginia Polytechnic Institute and State University
dc.relation.isformatofOCLC# 12763552
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1985.J426
dc.subject.lcshSwine -- Experiments
dc.subject.lcshSwine -- Physiology
dc.subject.lcshVeterinary obstetrics -- Experiments
dc.subject.lcshSwine -- Reproduction
dc.titleIn vitro progesterone and estrone synthesis by the porcine placenta and endometrium at 30, 60 and 90 days of gestation
dc.typeThesis
dc.contributor.departmentAnimal Science
dc.description.degreeM.S.
thesis.degree.nameM.S.
thesis.degree.levelmasters
thesis.degree.grantorVirginia Polytechnic Institute and State University
thesis.degree.disciplineAnimal Science
dc.type.dcmitypeText


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