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dc.contributor.authorLevin, Elenaen
dc.contributor.authorRaphael, Ginaten
dc.contributor.authorMa, Jingen
dc.contributor.authorBallester, Ana-Rosaen
dc.contributor.authorFeygenberg, Olegen
dc.contributor.authorNorelli, Johnen
dc.contributor.authorAly, Radien
dc.contributor.authorGonzalez-Candelas, Luisen
dc.contributor.authorWisniewski, Michaelen
dc.contributor.authorDroby, Samiren
dc.date.accessioned2019-07-08T11:48:40Zen
dc.date.available2019-07-08T11:48:40Zen
dc.date.issued2019-06-15en
dc.identifier.citationLevin, E.; Raphael, G.; Ma, J.; Ballester, A.-R.; Feygenberg, O.; Norelli, J.; Aly, R.; Gonzalez-Candelas, L.; Wisniewski, M.; Droby, S. Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum. Microorganisms 2019, 7, 175.en
dc.identifier.urihttp://hdl.handle.net/10919/91196en
dc.description.abstractPenicillium expansum is a major postharvest pathogen that infects different fruits, mainly through injuries inflicted during harvest or subsequent handling after harvest. Several effectors were suggested to mediate pathogenicity of P. expansum in fruit tissue. Among these effectors Nep1-like proteins (NLPs), produced by various microorganisms with different lifestyles, are known for their ability to induce necrosis in dicot plants and were shown to be involved in virulence of several plant-related pathogens. This study was aimed at the identification and functional characterization of two NLP genes found in the genome of P. expansum. The genes were designated Penlp1 and Penlp2 and were found to code type1 and type3 NLP respectively. Necrosis-inducing activity of the two proteins was demonstrated by transient expression in Nicotiana benthamiana leaves. While Penlp1 expression was induced during apple infection and in liquid culture, the highest level of Penlp2 expression was found in ungerminated spores. Deletion of Penlp1, but not Penlp2, resulted in reduced virulence on apples manifested by reduced rate of lesion development (disease severity).en
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.publisherMDPIen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectblue molden
dc.subjectappleen
dc.subjectNep1en
dc.subjectvirulenceen
dc.subjectpathogenicityen
dc.subjecteffectorsen
dc.titleIdentification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansumen
dc.typeArticle - Refereeden
dc.date.updated2019-06-28T06:37:16Zen
dc.description.versionPublished versionen
dc.title.serialMicroorganismsen
dc.identifier.doihttps://doi.org/10.3390/microorganisms7060175en
dc.type.dcmitypeTexten


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