Now showing items 1-13 of 13

    • Capturing Enveloped Viruses on Affinity Grids for Downstream Cryo- Electron Microscopy Applications 

      Kiss, G.; Chen, X. M.; Brindley, M. A.; Campbell, P.; Afonso, C. L.; Ke, Z. L.; Holl, J. M.; Guerrero-Ferreira, R. C.; Byrd-Leotis, L. A.; Steel, J.; Steinhauer, D. A.; Plemper, R. K.; Kelly, Deborah F.; Spearman, P. W.; Wright, E. R. (Cambridge University Press, 2014-02)
      Electron microscopy (EM), cryo-electron microscopy (cryo-EM), and cryo-electron tomography (cryo-ET) are essential techniques used for characterizing basic virus morphology and determining the three-dimensional structure ...
    • Casein Kinase 1 Epsilon Regulates Glioblastoma Cell Survival 

      Varghese, Robin T.; Young, Sarah; Pham, Lily; Liang, Yanping; Pridham, Kevin J.; Guo, Sujuan; Murphy, Susan; Kelly, Deborah F.; Sheng, Zhi (Nature, 2018-09-11)
      Glioblastoma is the most common malignant brain cancer with a dismal prognosis. The difficulty in treating glioblastoma is largely attributed to the lack of effective therapeutic targets. In our previous work, we identified ...
    • A microchip platform for structural oncology applications. 

      Winton, Carly E.; Gilmore, Brian L.; Demmert, Andrew C.; Karageorge, Vasilea; Sheng, Zhi; Kelly, Deborah F.
      Recent advances in the development of functional materials offer new tools to dissect human health and disease mechanisms. The use of tunable surfaces is especially appealing as substrates can be tailored to fit applications ...
    • Molecular Analysis of BRCA1 in Human Breast Cancer Cells Under Oxidative Stress 

      Gilmore, Brian L.; Liang, Yanping; Winton, Carly E.; Patel, Kaya; Karageorge, Vasilea; Varano, A. Cameron; Dearnaley, Wwilliam; Sheng, Zhi; Kelly, Deborah F. (Nature Publishing Group, 2017-03-06)
      The precise manner in which physical changes to the breast cancer susceptibility protein (BRCA1) affect its role in DNA repair events remain unclear. Indeed, cancer cells harboring mutations in BRCA1 suffer from genomic ...
    • Molecular structure and dimeric organization of the Notch extracellular domain as revealed by electron microscopy. 

      Kelly, Deborah F.; Lake, Robert J.; Middelkoop, Teije C.; Fan, Hua-Ying; Artavanis-Tsakonas, Spyros; Walz, Thomas (2010-05-07)
      BACKGROUND: The Notch receptor links cell fate decisions of one cell to that of the immediate cellular neighbor. In humans, malfunction of Notch signaling results in diseases and congenital disorders. Structural information ...
    • Molecular Surveillance of Viral Processes Using Silicon Nitride Membranes 

      Gilmore, Brian L.; Tanner, Justin R.; McKell, Allison O.; Boudreaux, Crystal E.; Dukes, Madeline J.; McDonald, Sarah M.; Kelly, Deborah F. (MDPI, 2013-03)
      Here we present new applications for silicon nitride (SiN) membranes to evaluate biological processes. We determined that 50-nanometer thin films of SiN produced from silicon wafers were sufficiently durable to bind active ...
    • A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease 

      Gilmore, Brian L.; Winton, Carly E.; Demmert, Andrew C.; Tanner, Justin R.; Bowman, Sam; Karageorge, Vasilea; Patel, Kaya; Sheng, Zhi; Kelly, Deborah F. (Springer Nature, 2015-09-23)
      We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select ...
    • Patient-derived glioblastoma stem cells respond differentially to targeted therapies 

      Kanabur, Pratik; Guo, Sujuan; Simonds, Gary S.; Kelly, Deborah F.; Gourdie, Robert G.; Verbridge, Scott; Sheng, Zhi (Impact Journals Llc, 2016-12-27)
      The dismal prognosis of glioblastoma is, at least in part, attributable to the difficulty in eradicating glioblastoma stem cells (GSCs). However, whether this difficulty is caused by the differential responses of GSCs to ...
    • A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications 

      Guo, Sujuan; Liang, Yanping; Murphy, Susan F.; Huang, Angela; Shen, Haihong; Kelly, Deborah F.; Sobrado, Pablo; Sheng, Zhi (Taylor & Francis Inc, 2015-03-01)
      The lack of a rapid and quantitative autophagy assay has substantially hindered the development and implementation of autophagy-targeting therapies for a variety of human diseases. To address this critical issue, we developed ...
    • Structural analysis of BRCA1 reveals modification hotspot 

      Liang, Yanping; Dearnaley, William J.; Varano, A. Cameron; Winton, Carly E.; Gilmore, Brian L.; Alden, Nick A.; Sheng, Zhi; Kelly, Deborah F. (Amer Assoc Advancement Science, 2017-09-01)
    • Survival kinase genes present prognostic significance in glioblastoma 

      Varghese, Robin T.; Liang, Yanping; Guan, Ting; Franck, Christopher T.; Kelly, Deborah F.; Sheng, Zhi (Impact Journals Llc, 2016-04-12)
      Cancer biomarkers with a strong predictive power for diagnosis/prognosis and a potential to be therapeutic targets have not yet been fully established. Here we employed a loss-of-function screen in glioblastoma (GBM), an ...
    • Visualizing Nanoparticle Mobility in Liquid at Atomic Resolution 

      Dukes, Madeline J.; Jacobs, Benjamin W.; Morgan, David G.; Hegde, Harshad; Kelly, Deborah F. (The Royal Society of Chemistry, 2013-02-28)
      Gold nanorods are widely known for their photothermal properties to treat solid tumors. Our work demonstrates the unrealized capacity to image these reagents in liquid at high resolution using Transmission Electron Microscopy ...
    • Visualizing virus particle mobility in liquid at the nanoscale 

      Varano, A. Cameron; Rahimi, Amina; Dukes, Madeline J.; Poelzing, Steven; McDonald, Sarah M.; Kelly, Deborah F. (The Royal Society of Chemistry, 2015-09-04)
      Currently, there remains a critical need to develop real-time imaging resources for life sciences. Here, we demonstrate the use of high resolution in situ imaging to observe biological complexes in liquid at the nanoscale. ...