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dc.contributor.authorBenton, Angela H.
dc.contributor.authorJackson, Mary Darby
dc.contributor.authorWong, Sandy M.
dc.contributor.authorDees, Justine L.
dc.contributor.authorAkerley, Brian J.
dc.contributor.authorMarquart, Mary E.
dc.date.accessioned2019-09-06T12:48:58Z
dc.date.available2019-09-06T12:48:58Z
dc.date.issued2019-08-24
dc.identifier.citationBenton, A.H.; Jackson, M.D.; Wong, S.M.; Dees, J.L.; Akerley, B.J.; Marquart, M.E. A Transcriptional Activator of Ascorbic Acid Transport in Streptococcus pneumoniae Is Required for Optimal Growth in Endophthalmitis in a Strain-Dependent Manner. Microorganisms 2019, 7, 290.
dc.identifier.urihttp://hdl.handle.net/10919/93409
dc.description.abstractStreptococcus pneumoniae is among the top causes of bacterial endophthalmitis, an infectious disease of the intraocular fluids. The mechanisms by which S. pneumoniae grows and thrives in the intraocular cavity are not well understood. We used a bacterial genome-wide assessment tool (transposon insertion site sequencing) to determine genes essential for S. pneumoniae growth in vitreous humor. The results indicated that an ascorbic acid (AA) transport system subunit was important for growth. We created an isogenic gene deletion mutant of the AA transcriptional activator, ulaR2, in 2 strains of S. pneumoniae. Growth curve analysis indicated that ulaR2 deletion caused attenuated growth in vitro for both strains. However, in vivo vitreous humor infection in rabbits with either strain determined that ulaR2 was necessary for growth in one strain but not the other. These results demonstrate that ulaR2 may be important for fitness during S. pneumoniae endophthalmitis depending on the background of the strain.en
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherMDPI
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA Transcriptional Activator of Ascorbic Acid Transport in Streptococcus pneumoniae Is Required for Optimal Growth in Endophthalmitis in a Strain-Dependent Manneren_US
dc.typeArticle - Refereed
dc.date.updated2019-09-06T10:06:53Z
dc.title.serialMicroorganisms
dc.identifier.doihttps://doi.org/10.3390/microorganisms7090290
dc.type.dcmitypeText


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Creative Commons Attribution 4.0 International (CC BY 4.0)
License: Creative Commons Attribution 4.0 International (CC BY 4.0)