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dc.contributor.authorTraore, Sy Mamadouen
dc.contributor.authorZhao, Bingyuen
dc.identifier.citationTraore and Zhao Plant Methods 2011, 7:42
dc.description.abstractBackground: Cloning genes into plasmid vectors is one of the key steps for studying gene function. Recently, Invitrogen™ developed a convenient Gateway® cloning system based on the site-specific DNA recombination properties of bacteriophage lambda and the cytotoxic protein ccdB, which is lethal to most E. coli strains. The ccdB protein, however, is not toxic to Agrobacterium tumefaciens, an important player often used for studying gene function in planta. This limits the direct application of the Gateway® cloning system in plant transformationmediated research. Results: In this study, we constructed a novel Gateway®-compatible destination vector, pEG101-SacB/R, by replacing the ccdB gene with a SacB-SacR gene cassette as the negative selectable marker. Conclusion: Our results demonstrate that the new pEG101-SacB/R destination vector can be used for Gateway® cloning in Agrobacterium tumefaciens. pEG101-SacB/R will be a valuable tool for high-throughput functional analysis of genes in planta.en
dc.description.sponsorshipFinancial support was provided by grants from Binational Agricultural Research and Development Fund (BARD) (US-4216-09 to BZ), US NSF (IOS- 0845283 to BZ), and the Virginia Agricultural Experiment Station (VA135872).en
dc.format.extent8 pagesen
dc.rightsCreative Commons Attribution Licenseen
dc.titleA novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciensen
dc.typeArticle - Refereeden
dc.title.serialPlant Methodsen

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License: Creative Commons Attribution License