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dc.contributor.authorGuza, Rebecca Clareen
dc.date.accessioned2011-08-06T16:01:45Zen
dc.date.available2011-08-06T16:01:45Zen
dc.date.issued2004-06-17en
dc.identifier.otheretd-06142004-174331en
dc.identifier.urihttp://hdl.handle.net/10919/9975en
dc.description.abstractAs part of the continuing work of the International Cooperative Biodiversity Group (ICBG), plant extracts were received from Madagascar. The extracts were screened for cytotoxicity using the A2780 human ovarian cancer cell line bioassay. The crude extract of Casearia nigrescens was fractionated and yielded five known compounds and one new compound that were cytotoxic. Mass spectrometry and 1D and 2D NMR techniques were used to determine the structure of the isolated compounds. The dichloromethane fraction of Casearia nigrescens was weakly active in the A2780 human ovarian cancer cell line bioassay. Further separation of the dichloromethane fraction resulted in the isolation of five known compounds (casearlucin A, caseamenbrol A, rel-18(S),19(R)-diacetoxy-18,19-epoxy-6(R)-hydroxy-2(S)-(2ξ-methylbutanoyloxy)-5(R),8(S),9(S),10(R)-cleroda-3,13(16),14-triene, casearlucin B, and rel-18(S),19(R)-diacetoxy-18,19-epoxy-2(S)-(2ξ-ethylbutanoyloxy)-5(R),8(S),9(S),10(R)-cleroda-3,13(16),14-triene) and one new compound (casearlucin L). Based on the available literature this was the first investigation of the natural products of Casearia nigrescens. The structure of the known compounds was determined by comparison of the NMR, MS, optical rotation, UV, and IR data with the data found in the literature. The structure of casearlucin L was determined by NMR data and comparison with the NMR data for similar known compounds.en
dc.format.mediumETDen
dc.publisherVirginia Techen
dc.relation.haspartNatProdThesis.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectCasearia nigrescensen
dc.subjectclerodane diterpenesen
dc.subjectnatural productsen
dc.titleIsolation of Natural Products from Casearia nigrescensen
dc.typeThesisen
dc.contributor.departmentChemistryen
dc.description.degreeMaster of Scienceen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelmastersen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.disciplineChemistryen
dc.contributor.committeechairKingston, David G. I.en
dc.contributor.committeememberCarlier, Paul R.en
dc.contributor.committeememberLong, Timothy E.en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06142004-174331en
dc.date.sdate2004-06-14en
dc.date.rdate2004-06-30en
dc.date.adate2004-06-30en


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