Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction

dc.contributor.authorLo, Terryen
dc.contributor.committeechairInzana, Thomas J.en
dc.contributor.committeememberWong, Eric A.en
dc.contributor.committeememberSriranganathan, Nammalwaren
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T20:52:09Zen
dc.date.adate1997-08-25en
dc.date.available2014-03-14T20:52:09Zen
dc.date.issued1997-07-31en
dc.date.rdate1997-08-25en
dc.date.sdate1997-07-31en
dc.description.abstractTraditional serologic assays of Actinobacillus pleuropeumoniae often have problems with cross-reactivity. To avoid the complications of antibody-antigen reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae and identify serotype 5 strains. Primers specific to the conserved capsular export region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the exception of serotype 4. A second set of primers specific to the unique capsular biosynthesis region of serotype 5 amplified a unique 1.1 kb band for serotype 5 only. The sensitivity of this assay was determined to be less than 100 colony forming units. This PCR assay enables us to detect A. pleuronpeumoniae and definitively distinguishes serotype 5 strains from other serotyes.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-71497-17302en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-71497-17302/en
dc.identifier.urihttp://hdl.handle.net/10919/36902en
dc.publisherVirginia Techen
dc.relation.haspartAt.pdfen
dc.relation.haspartvita.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectPCRen
dc.subjectmultiplexen
dc.subjectserotypingen
dc.subjectActinobacillus pleuropneumoniaeen
dc.titleDetection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reactionen
dc.typeThesisen
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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