Phylogenetic review of the millipede genus Cherokia Chamberlin, 1949 (Polydesmida, Xystodesmidae)

dc.contributor.authorVasquez-Valverde, Luisa Fernandaen
dc.contributor.authorMarek, Paul E.en
dc.description.abstractThe millipede genus Cherokia Chamberlin, 1949 is a monospecific taxon, with the type species Cherokia georgiana (Bollman, 1889). The last revision of the genus was made by Hoffman (1960) where he established three subspecies. Here we used molecular phylogenetics to assess the genus and evaluate whether it is a monophyletic group, and if the subspecies are each monophyletic. We included material from literature records and three natural history collections. Newly collected samples were obtained through a citizen science project. Morphological characters underlying subspecies groups???the shape of the paranota, body size, and coloration???were evaluated. A molecular phylogeny of the genus was estimated based on DNA sequences for seven gene loci, and a species delimitation analysis was used to evaluate the status of the subspecies. The documented geographical range of Cherokia in the United States was expanded to include a newly reported state record (Virginia) and about 160 new localities compared to the previously known range. Morphological characters, which included the shape of the paranota and body size that had been historically used to establish subspecies, showed clinal variation with a direct relationship with geographical distribution and elevation, but not with phylogeny. Coloration was highly variable and did not accord with geography or phylogeny. The phylogeny recovered Cherokia as a monophyletic lineage, and the species delimitation test supported the existence of a single species. The subspecies Cherokia georgiana ducilla (Chamberlin, 1939) and Cherokia georgiana latassa Hoffman, 1960 have been synonymized with Cherokia georgiana. The molecular and morphological evidence showed that Cherokia is a monospecific genus with the sole species, Cherokia georgiana, being geographically widespread and highly variable in its morphology.en
dc.description.notesThis research was supported by a National Science Foundation grant to P. Marek (Division of Environmental Biology, Systematics and Biodiversity Sciences #1916368). Derek Hennen and Jackson Means helped confirm identifications of Cherokia observations on iNaturalist. We thank those who provided specimens: Roger Birkhead, Sawyer Birkhead, Chris Eaton, Barbara Graham, Christina Fizer, Sherrie White, Jessica Clay, Tracey Muise and Raegan Rainey. Robin Andrews and Bill Shear served on FVV's thesis committee, and provided suggestions for earlier versions of the manuscript. Nesrine Akkari, Weixin Liu and Dragan Antic were reviewers who provided comments on previous versions of this paper.en
dc.description.sponsorshipNational Science Foundation [1916368]en
dc.description.versionPublished versionen
dc.publisherPensoft Publishersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.subjectCitizen scienceen
dc.subjectDNA barcodingen
dc.titlePhylogenetic review of the millipede genus Cherokia Chamberlin, 1949 (Polydesmida, Xystodesmidae)en
dc.typeArticle - Refereeden


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