Subsidy-stress responses of ecosystem functions along experimental freshwater salinity gradients

Abstract

Human activity is increasing salt concentrations in freshwaters worldwide, but effects of freshwater salinity gradients on biogeochemical cycling are less understood than in saline, brackish, or marine environments. Using controlled microcosm experiments, we characterized (1) short-term (one to five days) biogeochemical responses and (2) water column metabolism along a freshwater salinity gradient of multiple salt types. After one day, microcosms were oxic (4.48-7.40 mg O2 L-1) but became hypoxic (1.20-3.31 mg L-1) by day five. After one day in oxic conditions, microbial respiration in magnesium-, sodium-, and sea salt-based salinity treatments showed a subsidy-stress response, with respiration increasing by over 100% as salinity increased from 30 to 350-800 mu S cm-1. Conversely, respiration consistently increased along a calcium-based salinity gradient, peaking at 1500 mu S cm-1. By day five, an inverse subsidy-stress response was observed with elevated respiration at upper or lower ends of the gradient except for the magnesium treatment, which had the lowest respiration at the highest salinity. Calcium- and magnesium-based salinity treatments also caused considerable changes in phosphorus concentrations and C:P and N:P. In a separate experiment, microbial respiration and water column primary production also displayed subsidy-stress responses, but imbalances in effect sizes caused consistently declining net community production with increasing salinity. Collectively, our results establish that short-term exposure to different salt ion concentrations can enhance freshwater biogeochemical cycling at relatively low concentrations and alter resource stoichiometry. Furthermore, the nature of effects of freshwater salinization may also change with oxygen availability.