Trulove, Susan2015-10-292015-10-292009-10-22http://hdl.handle.net/10919/62444In the course of doing research on the mosquito-borne pathogens chikungunya virus (CHIKV) and o' nyong-nyong virus (ONNV), Virginia Tech researchers have discovered an inconvenient truth about an assay, strand-specific quantitative real-time PCR (ssqPCR), increasingly being used to detect and measure replicating viral RNA in infected cells and tissues. The method most labs are using for ssqPCR is unreliable.text/htmlIn CopyrightResearchPress releaseVirginia Tech. University Relations