Alexander, Lindsey S.Seabolt, Brynn S.Rhoads, Robert P.Stahl, Chad H.2017-01-122017-01-122012-06-012072-6643http://hdl.handle.net/10919/74290Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4) deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW) were pair-fed liquid diets that were either PO4 adequate (0.9% total P), supra-adequate (1.2% total P) in PO4 requirement or deficient (0.7% total P) in PO4 content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured in vitro for 7 days to determine if PO4 nutrition alters their ability to proceed through their myogenic lineage. Dietary PO4 deficiency resulted in reduced (P < 0.05) sera PO4 and parathyroid hormone (PTH) concentrations, while supra-adequate dietary PO4 improved (P < 0.05) feed conversion efficiency as compared to the PO4 adequate group. In vivo satellite cell proliferation was reduced (P < 0.05) among the PO4 deficient pigs, and these cells had altered in vitro expression of markers of myogenic progression. Further work to better understand early nutritional programming of satellite cells and the potential benefits of emphasizing early PO4 nutrition for future lean growth potential is warranted.436 - 448 (13) page(s)application/pdfenCreative Commons Attribution 4.0 InternationalNutrition & Dieteticssatellite cellphosphateneonatalpigSKELETAL-MUSCLE GROWTHPROLIFERATIONDIFFERENTIATIONDYNAMICSNUCLEITURKEYRATSArticle - Refereedhttps://doi.org/10.3390/nu406043646