Lindsay, David S.Lenz, S. D.Blagburn, B. L.Brake, D. A.2014-06-202014-06-201999-02David S. Lindsay, Steve D. Lenz, Byron L. Blagburn and David A. Brake (1999). "Characterization of temperature-sensitive strains of Neospora caninum in mice," The Journal of Parasitology, Vol. 85, No. 1, pp. 64-67. DOI: 10.2307/32857010022-3395http://hdl.handle.net/10919/49028Temperature-sensitive (ts) strains of the Neospora caninum tachyzoites were selected by chemical mutagenesis and selection for growth at 32 C. Three ts strains and the parental, N. caninum wild-type strain, NC-1, were examined in the present study for their ability to cause disease in inbred BALB/c mice, outbred ICR mice, and chemically immunosuppressed ICR mice. In BALB/c mice, all 3 strains failed to induce clinical disease, whereas infection with the NC-1 strain caused central nervous system disease and death in some mice. No disease was observed in ICR mice inoculated with the 3 ts strains or the NC-I strain. All immunosuppressed ICR mice inoculated with the NC-1 strain died, whereas no immunosuppressed mice inoculated with the NCts-4 strain and only 1 of 5 mice inoculated with the NCts-8 and NCts-12 strains died. The NCts-4 and NCts-12 strains reverted to a wild-type phenotype when grown at 37 C. Vaccination of BALB/c mice with live, but not frozen NCts-8 strain tachyzoites induced significant (P < 0.05) protection following NC-1 strain challenge.application/pdfenIn Copyrights48 toxoplasma-gondiikilled vaccinesheepchallengepathogenicitytachyzoitesinfectionsimmunityhamstersmutantsparasitologyArticle - Refereedhttp://www.jstor.org/stable/3285701https://doi.org/10.2307/3285701