Tabor, Kathryn M.Marquart, Gregory D.Hurt, ChristopherSmith, Trevor S.Geoca, Alexandra K.Bhandiwad, Ashwin A.Subedi, AbhignyaSinclair, Jennifer L.Rose, Hannah M.Polys, Nicholas F.Burgess, Harold A.2019-10-182019-10-182019-02-082050-084Xe42687http://hdl.handle.net/10919/94637Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.application/pdfenCreative Commons Attribution 4.0 Internationalin-vivogene-expressionoptical controlknock-ingal4dissectionsystemarchitecturepatternstoolsArticle - Refereedhttps://doi.org/10.7554/eLife.42687830735129