2016-08-242016-08-242012-07-03http://hdl.handle.net/10919/72683The present invention comprises an in vitro enzymatic process that effectively converts renewable polysaccharides into high yields of hydrogen at mild conditions, using only enzymes and water. The process comprises a number of enzymes: (1) phosphorylases, (2) phosphoglucomutases, (3) hydrogenases, and (4) enzymes involved in the pentose-phosphate pathway. Preferred embodiments of the process produce only hydrogen and carbon dioxide as net products, translating into an inexpensive method of generating hydrogen in very large quantities from low-cost feedstocks.application/pdfen-USPatenthttp://pimg-fpiw.uspto.gov/fdd/81/116/082/0.pdf11747496423/648.1429/2435/166435/168C12P3/00H01M8/16Y02E60/527Y02P70/568211681