Greer-Short, Amara D.Poelzing, Steven2017-02-032017-02-032015-02-181664-042Xhttp://hdl.handle.net/10919/74924Rapid ventricular pacing rates induces two types of beats following pacing cessation: recovery cycle length (RCL) prolongation (overdrive suppression) and RCL shortening (overdrive excitation). The goals of this study were to compare common experimental protocols for studying triggered activity in whole-heart preparations and differentiate between recovery beats using a new methodology. Post-pacing recovery beat cycle length (RCL) and QRS were normalized to pre-paced R-R and QRS intervals and analyzed using a K-means clustering algorithm. Control hearts only produced suppressed beats: RCL ratio increased with rapid pacing (25 +/- 4.0%, n = 10) without changing QRS duration. Rapid pacing during hypercalcemia + hypothermia (5.5 mM and 34 degrees C) produced significantly earlier excited beats (53 +/- 14%, n = 5) with wider QRS durations (58 +/- 6.3%, n = 5) than suppressed beats. Digoxin + hypothermia (0.75 mu M) produced the most excited beats with significantly earlier RCL (44 +/- 3.2%, n = 6) and wider QRS (60 +/- 3.1%, n = 6) ratios relative to suppressed beats. Increasing pacing further shortened RCL (30 +/- 7.8%, n = 6). In a prospective study, TTX (100 nM) increased RCL ratio (15 +/- 6.0%, n = 10) without changing the QRS duration of excited beats. The algorithm was compared to a cross-correlation analysis with 93% sensitivity and 94% specificity. This ECG based algorithm distinguishes between triggered and automatic activity.10 pagesapplication/pdfenCreative Commons Attribution 4.0 InternationalPhysiologyautomaticitytriggered activityoverdrive excitationoverdrive suppressionhypothermiadigoxinhypercalcemiaCARDIAC PURKINJE-FIBERSDELAYED AFTERDEPOLARIZATIONSTRANSMEMBRANE POTENTIALSCALCIUM-CONCENTRATIONTRIGGERED ACTIVITYCANINE HEARTNA+ CHANNELSWHOLE HEARTIN-VIVOARRHYTHMIASArticle - Refereedhttps://doi.org/10.3389/fphys.2015.000146