Virginia TechWang, X. W.Cooper, K. L.Wang, AnboXu, J. C.Wang, Z. A.Zhang, Y.Tu, Zhijian Jake2014-04-162014-04-162006-10-01Wang, Xingwei; Cooper, Kristie L.; Wang, Anbo; et al., "Label-free DNA sequence detection using oligonucleotide functionalized optical fiber," Appl. Phys. Lett. 89, 163901 (2006); http://dx.doi.org/10.1063/1.23644590003-6951http://hdl.handle.net/10919/47385The authors present a label-free method for direct detection of deoxyribonucleic acid (DNA) sequences. The capture DNA is immobilized onto the surface of a silica optical fiber tip by means of the layer-by-layer electrostatic self-assembly technique. Hybridization of target DNA with complementary capture DNA increases the optical thickness of the fiber tip. This phenomenon can be detected by demodulation of the spectrum of a Fabry-Perot cavity fabricated in the optical fiber. Experimental results demonstrate sequence specificity and sensitivity to nanogram quantities of target DNA sequences with short (similar to 5 min) hybridization time. (c) 2006 American Institute of Physics.application/pdfenIn CopyrightBiosensorsArticle - Refereedhttp://scitation.aip.org/content/aip/journal/apl/89/16/10.1063/1.2364459https://doi.org/10.1063/1.2364459