2016-08-242016-08-241999-11-02http://hdl.handle.net/10919/72401Relating to chromatographic processes and ion-exchange and affinity matrices, a spatial installation method for a bifunctional reagent that crosslinks and/or activates a polymer matrix is disclosed, with inside-outside installation of a bifunctional reagent on and within a polymer matrix. The polymer matrix is cellulose, agarose, or chitosan particles. The installation may be followed by inside-outside ligand attachment, by further reacting the matrix with a ligand or ionic group so that a higher concentration of ligand or ionic moiety occurs on the intra-particle volume than the outer matrix surface.application/pdfen-USPatenthttp://pimg-fpiw.uspto.gov/fdd/45/773/059/0.pdf9033686210/198.2210/502.1210/635210/656502/404536/5796/101977/788B01J20/291B01D15/361B01D15/3804B01D15/3809B01J20/26B01J20/3212B01J20/3248B01J20/3274B01J2220/54Y10S977/7885977345