2019-02-262019-02-262017-03-21http://hdl.handle.net/10919/87801The present invention provides engineered tissue scaffolds, engineered tissues, and methods of using them. The scaffolds and tissues are derived from natural tissues and are created using non-thermal irreversible electroporation (IRE). Use of IRE allows for ablation of cells of the tissue to be treated, but allows vascular and neural structures to remain essentially unharmed. Use of IRE thus permits preparation of thick tissue scaffolds and tissues due to the presence of vasculature within the scaffolds. The engineered tissues can be used in methods of treating subjects, such as those in need of tissue replacement or augmentation.application/pdfen-USPatenthttp://pimg-fpiw.uspto.gov/fdd/91/986/095/0.pdf12432295A61L27/3691A61K35/12A61L27/3604A61L27/3625A61L27/3633A61L27/38A61L2430/28A61L2430/40C12N5/0068C12N13/00C12N2533/909598691