Matarneh, Sulaiman K.2017-11-092017-11-092017-07-12vt_gsexam:12491http://hdl.handle.net/10919/80033During postmortem metabolism, hydrogen ions accumulate in the muscle and gradually lower the pH from 7.2 to an ultimate pH near 5.6. The ultimate pH of meat is widely valued as an indicator of fresh meat quality as it directly affects the quality characteristics of color, texture, and water holding capacity. Therefore, our research was conducted to identify the processes responsible for determining ultimate pH. Pigs harboring the AMPK�•3R200Q mutation produce meat with extremely low ultimate pH (pH ~ 5.3) that is detrimental to quality. This phenomenon is often attributed to a greater glycogen content in muscle from the mutant pigs compared to wild-type pigs. However, our research indicated that greater glycolytic flux in muscle from these pigs causes a lower ultimate pH rather than greater tissue glycogen deposition. On the other hand, however, AMPK�•3R200Q pigs contain more mitochondria and retain greater oxidative capacity. Hence, we hypothesized that mitochondria may contribute to the lower ultimate pH in muscle of these pigs. To test our hypothesis, isolated mitochondria were incorporated into an in vitro system the mimics postmortem glycolysis. Mitochondria enhanced glycolytic flux and pH decline in the in vitro system similar to that of AMPK�•3R200Q pigs. After a series of experiments, we found that the causative agent for enhanced glycolytic flux is a soluble mitochondrial protein. In other experiments, mitochondrial F1-ATPase was found to be responsible for the majority of this effect, principally through promoting greater ATP hydrolysis at lower pH values, thereby allowing for greater flux through glycolysis. These data suggest that variations in ultimate pH may be more thoroughly explained and predicted by the abundance of mitochondria. Broiler pectoralis major muscle, which is a highly glycolytic muscle, possesses high ultimate pH (pH ~ 5.9) compared to pork and beef. We postulated that rapid carcass chilling reduces the flux through glycolysis, thereby causing premature termination of postmortem metabolism. Yet, chilling was only partially responsible for the high ultimate pH of pectoralis major muscle. However, we showed that pectoralis major of broiler chicken exhibits lower phosphofructokinase-1 activity compared to porcine longissimus lumborum muscle, which limits the flux through glycolysis.ETDen-USIn Copyrightmeat qualityultimate pHmitochondriaAMPKy3R200QDissertation