Caneschi, Washington LuizSanchez, Angelica BianchiniFelestrino, Erica Barbosade Carvalho Lemes, Camila GracyelleCordeiro, Isabella FerreiraFonseca, Natasha PeixotoVilla, Morghana MarinaVieira, Izadora TabusoGoncalves Moraes, Lauro AngeloBarbosa Assis, Renata de Almeidado Carmo, Flavio FonsecaYoshino Kamino, Luciana HiromiSilva, Robson SoaresFerro, Jesus AparecidoTiraboschi Ferro, Maria InesFerreira, Rafael MariniSantos, Vera LuciaMourao Silva, Ubiana de CassiaAlmeida, Nalvo F.Varani, Alessandro de MelloMachado Garcia, Camila CarriaoSetubal, Joao CarlosMoreira, Leandro Marcio2020-02-052020-02-052019-11-292045-232218006http://hdl.handle.net/10919/96718Serratia liquefaciens strain FG3 (SlFG3), isolated from the flower of Stachytarpheta glabra in the Brazilian ferruginous fields, has distinctive genomic, adaptive, and biotechnological potential. Herein, using a combination of genomics and molecular approaches, we unlocked the evolution of the adaptive traits acquired by S1FG3, which exhibits the second largest chromosome containing the largest conjugative plasmids described for Serratia. Comparative analysis revealed the presence of 18 genomic islands and 311 unique protein families involved in distinct adaptive features. S1FG3 has a diversified repertoire of genes associated with Nonribosomal peptides (NRPs/PKS), a complete and functional cluster related to cellulose synthesis, and an extensive and functional repertoire of oxidative metabolism genes. In addition, S1FG3 possesses a complete pathway related to protocatecuate and chloroaromatic degradation, and a complete repertoire of genes related to DNA repair and protection that includes mechanisms related to UV light tolerance, redox process resistance, and a laterally acquired capacity to protect DNA using phosphorothioation. These findings summarize that SlFG3 is well-adapted to different biotic and abiotic stress situations imposed by extreme conditions associated with ferruginous fields, unlocking the impact of the lateral gene transfer to adjust the genome for extreme environments, and providing insight into the evolution of prokaryotes.application/pdfenCreative Commons Attribution 4.0 InternationalArticle - Refereedhttps://doi.org/10.1038/s41598-019-54601-4931784663