Xu, ChangHuang, XinMa, NingLiu, YanrongXu, AijiaoZhang, XunzhongLi, DayongLi, YueZhang, WanjunWang, Kehua2024-05-102024-05-102024-04-30Xu, C.; Huang, X.; Ma, N.; Liu, Y.; Xu, A.; Zhang, X.; Li, D.; Li, Y.; Zhang, W.; Wang, K. MicroRNA164 Affects Plant Responses to UV Radiation in Perennial Ryegrass. Plants 2024, 13, 1242.https://hdl.handle.net/10919/118952Increasing the ultraviolet radiation (UV) level, particularly UV-B due to damage to the stratospheric ozone layer by human activities, has huge negative effects on plant and animal metabolism. As a widely grown cool-season forage grass and turfgrass in the world, perennial ryegrass (<i>Lolium perenne</i>) is UV-B-sensitive. To study the effects of miR164, a highly conserved microRNA in plants, on perennial ryegrass under UV stress, both Os<i>miR164</i>a overexpression (OE164) and target mimicry (MIM164) transgenic perennial ryegrass plants were generated using agrobacterium-mediated transformation, and UV-B treatment (~600 &mu;w cm^&minus;2) of 7 days was imposed. Morphological and physiological analysis showed that the <i>miR164</i> gene affected perennial ryegrass UV tolerance negatively, demonstrated by the more scorching leaves, higher leaf electrolyte leakage, and lower relative water content in OE164 than the WT and MIM164 plants after UV stress. The increased UV sensitivity could be partially due to the reduction in antioxidative capacity and the accumulation of anthocyanins. This study indicated the potential of targeting <i>miR164</i> and/or its targeted genes for the genetic manipulation of UV responses in forage grasses/turfgrasses; further research to reveal the molecular mechanism underlying how miR164 affects plant UV responses is needed.application/pdfenCreative Commons Attribution 4.0 InternationalArticle - Refereed2024-05-10https://doi.org/10.3390/plants13091242