Sandal, IndraBhattacharya, AmitaSaini, UkshaKaur, DevinderSharma, ShvetaGulati, AshuKumar, Jonnala K.Kumar, NeerajDayma, JyotsnaDas, PralaySingh, BikramAhuja, Paramvir S.2012-08-242012-08-242011-05-31BMC Chemical Biology. 2011 May 31;11(1):1http://hdl.handle.net/10919/18806Background Accidental autoclaving of L-glutamine was found to facilitate the Agrobacterium infection of a non host plant like tea in an earlier study. In the present communication, we elucidate the structural changes in L-glutamine due to autoclaving and also confirm the role of heat transformed L-glutamine in Agrobacterium mediated genetic transformation of host/non host plants. Results When autoclaved at 121°C and 15 psi for 20 or 40 min, L-glutamine was structurally modified into 5-oxo proline and 3-amino glutarimide (α-amino glutarimide), respectively. Of the two autoclaved products, only α-amino glutarimide facilitated Agrobacterium infection of a number of resistant to susceptible plants. However, the compound did not have any vir gene inducing property. Conclusions We report a one pot autoclave process for the synthesis of 5-oxo proline and α-amino glutarimide from L-glutamine. Xenobiotic detoxifying property of α-amino glutarimide is also proposed.application/pdfenCreative Commons Attribution 4.0 InternationalArticle - Refereed2012-08-24Indra Sandal et al.; licensee BioMed Central Ltd.https://doi.org/10.1186/1472-6769-11-1