Waitt, Jessie Anne2013-05-222013-05-222013-05-21vt_gsexam:859http://hdl.handle.net/10919/22078In the United States, illnesses associated with fresh produce are increasing in frequency.  While contamination risks are present at every aspect of the farm to fork continuum, post-harvest practices holds the potential for cross-contamination of large amounts of product.  Post-harvest contamination risks for hydroponically grown lettuce packaged with intact roots and sold as "living lettuce"" are poorly understood.  In this study, transmission of Salmonella enterica serotype Enteritidis to the roots and leaves of butterhead lettuce was studied when contamination was introduced during typical handling practices.  The effectiveness of random sampling strategies for selection of Salmonella contaminated leaves was assessed by co-inoculating the Salmonella solution with Glo Germ™ and comparing recovery from blacklight selected leaves.  The recovery of Salmonella was improved by only 0.5 log CFU/g when blacklight was used to select Glo Germ™ contaminated leaves (P=0.05). This suggests random leaf selection as described by current FDA protocols is adequate. In addition, this study showed rapid transfer of Salmonella from liquid to the roots and sub-sequentially to the leaves of living lettuce.  Salmonella persisted but did not grow on leaves when stored at 4˚C for 18-days. Storage at 12˚C was associated with 2 log CFU/g increases in Salmonella on roots after 18-days storage (P=0.0002), while 4˚C storage was associated with a decrease of 0.4 log CFU/g Salmonella on roots (P=0.0001). Growth occurred only under temperature abuse conditions.  This reinforces the need for maintaining temperature control and highlights the importance of identifying risks associated with post-harvest handling during hydroponic production and distribution.ETDIn CopyrightLiving lettuceSalmonella entericahydroponic productionThesis