Holder, Amara L.Marr, Linsey C.2017-02-052017-02-052013-01-01Amara L. Holder, Linsey C. Marr, "Toxicity of Silver Nanoparticles at the Air-Liquid Interface", BioMed Research International, vol. 2013, Article ID 328934, 11 pages, 2013. https://doi.org/10.1155/2013/3289342314-6133http://hdl.handle.net/10919/74935Silver nanoparticles are one of the most prevalent nanomaterials in consumer products. Some of these products are likely to be aerosolized, making silver nanoparticles a high priority for inhalation toxicity assessment. To study the inhalation toxicity of silver nanoparticles, we have exposed cultured lung cells to them at the air-liquid interface. Cells were exposed to suspensions of silver or nickel oxide (positive control) nanoparticles at concentrations of 2.6, 6.6, and 13.2 μg cm⁻² (volume concentrations of 10, 25, and 50 μg ml⁻¹) and to 0.7 μg cm⁻² silver or 2.1 μg cm⁻² nickel oxide aerosol at the air-liquid interface. Unlike a number of in vitro studies employing suspensions of silver nanoparticles, which have shown strong toxic effects, both suspensions and aerosolized nanoparticles caused negligible cytotoxicity and only a mild inflammatory response, in agreement with animal exposures. Additionally, we have eveloped a novel method using a differential mobility analyzer to select aerosolized nanoparticles of a single diameter to assess the size-dependent toxicity of silver nanoparticles.? - ? (11) page(s)application/pdfenCreative Commons Attribution 4.0 InternationalBiotechnology & Applied MicrobiologyMedicine, Research & ExperimentalResearch & Experimental MedicineHUMAN HEPATOMA-CELLSSPRAGUE-DAWLEY RATSHUMAN LUNG-CELLSIN-VITROPARTICULATE MATTERINHALATION TOXICITYEPITHELIAL-CELLSEXPOSURE SYSTEMDIESEL EXHAUSTPARTICLESArticle - Refereedhttps://doi.org/10.1155/2013/328934