2016-08-242016-08-242013-09-24http://hdl.handle.net/10919/72703Pythium, when cultured with crude glycerol as a carbon source, produces useful polyunsaturated fatty acids such as eicosapentaenoic acid (EPA). The crude glycerol is pretreated to remove soaps and methanol. An exemplary Pythium species for use in the production of EPA is Pythium irregulare.application/pdfen-USPatenthttp://pimg-fpiw.uspto.gov/fdd/10/412/085/0.pdf13486464435/134435/171C12P7/6472C12P7/64278541210