Virginia Tech. Department of MathematicsVirginia Bioinformatics InstituteArat, SedaBullerjahn, George S.Laubenbacher, Reinhard C.2015-10-012015-10-012015-02-23Arat, S., Bullerjahn, G. S., & Laubenbacher, R. (2015). A Network Biology Approach to Denitrification in Pseudomonas Aeruginosa. Plos One, 10(2), e0118235. doi: 10.1371/journal.pone.01182351932-6203http://hdl.handle.net/10919/56683Pseudomonas aeruginosa is a metabolically flexible member of the Gammaproteobacteria. Under anaerobic conditions and the presence of nitrate, P. aeruginosa can perform (complete) denitrification, a respiratory process of dissimilatory nitrate reduction to nitrogen gas via nitrite (NO₂), nitric oxide (NO) and nitrous oxide (N₂O). This study focuses on understanding the influence of environmental conditions on bacterial denitrification performance, using a mathematical model of a metabolic network in P. aeruginosa. To our knowledge, this is the first mathematical model of denitrification for this bacterium. Analysis of the long-term behavior of the network under changing concentration levels of oxygen (O₂), nitrate (NO₃), and phosphate (PO₄) suggests that PO₄ concentration strongly affects denitrification performance. The model provides three predictions on denitrification activity of P. aeruginosa under various environmental conditions, and these predictions are either experimentally validated or supported by pertinent biological literature. One motivation for this study is to capture the effect of PO₄ on a denitrification metabolic network of P. aeruginosa in order to shed light on mechanisms for greenhouse gas N₂O accumulation during seasonal oxygen depletion in aquatic environments such as Lake Erie (Laurentian Great Lakes, USA). Simulating the microbial production of greenhouse gases in anaerobic aquatic systems such as Lake Erie allows a deeper understanding of the contributing environmental effects that will inform studies on, and remediation strategies for, other hypoxic sites worldwide.12 pagesapplication/pdfen-USIn CopyrightAlgebraBacteriaDiscrete modelsDissolved substancesEscherichia-coliGeneLake erieNitrous-oxide reductasePhosphatePhosphorusArticle - Refereedhttps://doi.org/10.1371/journal.pone.0118235102